beta-trace gene expression is regulated by a core promoter and a distal thyroid hormone response element

We isolated and characterized the human P-Trace protein (beta TP) gene promoter. beta TP, also known as prostaglandin D-2 synthase, is a lipocalin secreted from the choroid plexus and meninges into cerebrospinal fluid. Basal transcription of the beta TP gene is directed from a core promoter found within the first 325 bases of the 5'-flanking sequence. The beta TP gene promoter is responsive to thyroid hormone (3,3',5 triiodothyronine, T-3) and efficiently repressed by unliganded human thyroid hormone receptor beta (TR beta, Functional analysis of the beta TP promoter in TE671 cells revealed that responsiveness to T-3 occurs in sequences 2.5 kilobase pairs 5' of the start site, Within the hormone-responsive region we identified a thyroid hormone response element (TRE) located from -2576 to -2562 base pairs relative to the transcription start site. The beta TP TRE is composed of two directly repeated consensus half-sites separated by a 3-base pair space (DR3). The beta TP TRE forms specific complexes with TR beta, We have shown that a gene active in the choroid plexus and meninges is responsive to T-3. T-3 may play a role in the regulated transport of substances into the cerebrospinal fluid and ultimately the brain.