Involvement of the ammonium transporter AmtB in nitrogenase regulation and ammonium excretion in Pseudomonas stutzeri A1501

被引：39

作者：

Zhang, Tao ^{[1
,2
]}

Yan, Yongliang ^{[1
]}

He, Sheng ^{[1
]}

Ping, Shuzhen ^{[1
]}

Alam, Khandakar Mohiul ^{[1
]}

Han, Yunlei ^{[1
]}

Liu, Xiaodong ^{[1
]}

Lu, Wei ^{[1
]}

Zhang, Wei ^{[1
]}

Chen, Ming ^{[1
]}

Xiang, Wensheng ^{[2
]}

Wang, Xiangjing ^{[2
]}

Lin, Min ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Key Lab Crop Biotechnol, Minist Agr, Biotechnol Res Inst, Beijing 100081, Peoples R China

[2] NE Agr Univ, Sch Life Sci, Harbin 150030, Peoples R China

来源：

RESEARCH IN MICROBIOLOGY | 2012年 / 163卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Pseudomonas stutzeri; Ammonium transporter AmtB; Nitrogenase regulation; Ammonium excretion; DEPENDENT MEMBRANE SEQUESTRATION; COMPLETE GENOME SEQUENCE; P-II PROTEIN; ESCHERICHIA-COLI; POSTTRANSLATIONAL REGULATION; AZOSPIRILLUM-BRASILENSE; ADP-RIBOSYLATION; CHANNEL AMTB; GLNK; STRAIN;

D O I：

10.1016/j.resmic.2012.05.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nitrogen-fixing Pseudomonas stutzeri strain A1501 contains two ammonium transporter genes, amtB1 and amtB2, linked to glnK. Growth of an amtB1-amtB2 double deletion mutant strain was not impaired compared to that of the wild type under any conditions tested, and it was still capable of taking up ammonium ions at nearly wild-type rates. Nitrogenase activity was repressed in wild-type strain A1501 in response to the addition of ammonium, but nitrogenase activity was only partially impaired in the amtB1 and amtB2 double mutant, suggesting that the two AmtB proteins are involved in regulating expression of nitrogenase or its activity in response to ammonium. An interaction between GlnK and AmtB1 or AmtB2 was observed in a yeast two-hybrid assay. Ammonium was excreted by the amtB double mutant strain under nitrogen fixation conditions, particularly when nifA was expressed constitutively. This suggests that AmtB proteins play a role in controlling the internal pool of ammonia within the cell. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

引用

页码：332 / 339

页数：8

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