NMDA receptors and PSD-95 are found in attachment plaques in cerebellar granular layer glomeruli

被引:34
作者
Petralia, RS [1 ]
Wang, YX [1 ]
Wenthold, RJ [1 ]
机构
[1] NIDCD, Neurochem Lab, NIH, Bethesda, MD 20892 USA
关键词
AMPA; cadherin; catenin; contactus acherens; puncta adherentia;
D O I
10.1046/j.1460-9568.2002.01896.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
N-methyl-D-aspartate (NMDA) receptors mediate long-term changes in excitatory synapses in response to glutamate release. In the cerebellar granular layer, most glutamatergic synapses are formed between mossy terminals and granule cell dendrites, which together with some other components, make up complex glomerular structures. Glomeruli contain numerous attachment plaques (or puncta adherentia), which are sites of adhesion between cells. These structures are found mainly between granule cell dendrites, and probably help maintain the integrity of glomeruli. Attachment plaques contain adhesive proteins such as cadherins. In this study, we show that NMDA receptors are common at these attachment plaques, in addition to being found at synapses. We used four different antibodies to the NMDA receptor subunit, NR1, and another to NR2A/B. In contrast, labelling for an alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) glutamate receptor antibody was seen only in a few attachment plaques, although AMPA receptors were seen frequently at glomerular synapses. We also show that substantial levels of the NMDA receptor-associated protein, PSD-95, are found in both synapses and attachment plaques. One way that NMDA receptors mediate changes in synapses is through effects on synaptic cadherins, which change their adhesive properties in response to NMDA receptor activation and consequently may alter synaptic function. The presence of NMDA receptors in attachment plaques suggests that these receptors mediate changes in the adhesive properties of these plaques, similar to this function in synapses.
引用
收藏
页码:583 / 587
页数:5
相关论文
共 45 条
  • [1] DIFFERENTIAL EXPRESSION OF 5 N-METHYL-D-ASPARTATE RECEPTOR SUBUNIT MESSENGER-RNAS IN THE CEREBELLUM OF DEVELOPING-RATS AND ADULT-RATS
    AKAZAWA, C
    SHIGEMOTO, R
    BESSHO, Y
    NAKANISHI, S
    MIZUNO, N
    [J]. JOURNAL OF COMPARATIVE NEUROLOGY, 1994, 347 (01) : 150 - 160
  • [2] Long-term potentiation of intrinsic excitability at the Mossy fiber-granule cell synapse of rat cerebellum
    Armano, S
    Rossi, P
    Taglietti, V
    D'Angelo, E
    [J]. JOURNAL OF NEUROSCIENCE, 2000, 20 (14) : 5208 - 5216
  • [3] BahjaouiBouhaddi M, 1997, J COMP NEUROL, V378, P180, DOI 10.1002/(SICI)1096-9861(19970210)378:2<180::AID-CNE3>3.0.CO
  • [4] 2-#
  • [5] Relationship between N-methyl-D-aspartate receptor NR1 splice variants and NR2 subunits
    Blahos, J
    Wenthold, RJ
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (26) : 15669 - 15674
  • [6] Stargazin regulates synaptic targeting of AMPA receptors by two distinct mechanisms
    Chen, L
    Chetkovich, DM
    Petralia, RS
    Sweeney, NT
    Kawasaki, Y
    Wenthold, RJ
    Bredt, DS
    Nicoll, RA
    [J]. NATURE, 2000, 408 (6815) : 936 - 943
  • [7] DIGREGORIO DA, 2001, SOC NEUR ABSTR, V27
  • [8] Dingledine R, 1999, PHARMACOL REV, V51, P7
  • [9] Hamori Jozsef, 1997, Journal of Neural Transplantation and Plasticity, V6, P11
  • [10] Blockade of cadherin-6B activity perturbs the distribution of PSD-95 family proteins in retinal neurones
    Honjo, Y
    Nakagawa, S
    Takeichi, M
    [J]. GENES TO CELLS, 2000, 5 (04) : 309 - 318