Interferon-inducible protein-10 involves vascular smooth muscle cell migration, proliferation, and inflammatory response

被引:133
作者
Wang, XK
Yue, TL
Ohlstein, EH
Sung, CP
Feuerstein, GZ
机构
[1] Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia
[2] Dept. of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia
关键词
D O I
10.1074/jbc.271.39.24286
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Interferon-inducible protein-10 (IP-10) is a member of the C-X-C chemokine family. Using mRNA differential display, we isolated a rat homologue to murine and human IP-10 from lipopolysaccharide-stimulated carotid arteries. Our studies demonstrated that IP-10 is a potent mitogenic and chemotactic factor for vascular smooth muscle cells, the critical features of smooth muscle cells for their contribution to the pathogenesis of atherosclerosis and restenosis. IP-10 induced a concentration-dependent stimulation of DNA synthesis, cell proliferation, and cell migration of rat aortic smooth muscle cells. A concentration- and time-dependent IP-10 mRNA induction was observed in lipopolysaccharide- or interferon-gamma-stimulated, but not interleukin-1 beta- or tumor necrosis factor-alpha-stimulated smooth muscle cells. A marked synergistic effect on IP-10 mRNA expression was observed when smooth muscle cells were challenged with interferon-gamma together with interleukin-1 beta or tumor necrosis factor-alpha. Furthermore, IP-10 mRNA expression was induced in the rat carotid artery after balloon angioplasty. The mitogenic and chemotactic features of IP-10 for smooth muscle cells, along with its discrete induction in cultured vascular smooth muscle cells and in carotid arteries after balloon angioplasty (neointima formation) suggest that IP-10 may play an active and distinct role in vascular remodeling processes.
引用
收藏
页码:24286 / 24293
页数:8
相关论文
共 40 条
[1]   CYTOKINE-INDUCED EXPRESSION OF MESSENGER-RNAS FOR CHEMOTACTIC FACTORS IN HUMAN SYNOVIAL-CELLS AND FIBROBLASTS [J].
BEDARD, PA ;
GOLDS, EE .
JOURNAL OF CELLULAR PHYSIOLOGY, 1993, 154 (02) :433-441
[2]  
BEVILACQUA MP, 1993, ANNU REV IMMUNOL, V11, P767, DOI 10.1146/annurev.iy.11.040193.004003
[3]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[4]  
CLINTON SK, 1992, ARCH PATHOL LAB MED, V116, P1292
[5]   THE EXPRESSION OF THE ADHESION MOLECULES ICAM-1, VCAM-1, PECAM, AND E-SELECTIN IN HUMAN ATHEROSCLEROSIS [J].
DAVIES, MJ ;
GORDON, JL ;
GEARING, AJH ;
PIGOTT, R ;
WOOLF, N ;
KATZ, D ;
KYRIAKOPOULOS, A .
JOURNAL OF PATHOLOGY, 1993, 171 (03) :223-229
[6]   IP-10, A GAMMA-INTERFERON-INDUCIBLE PROTEIN RELATED TO INTERLEUKIN-8, LACKS NEUTROPHIL ACTIVATING PROPERTIES [J].
DEWALD, B ;
MOSER, B ;
BARELLA, L ;
SCHUMACHER, C ;
BAGGIOLINI, M ;
CLARKLEWIS, I .
IMMUNOLOGY LETTERS, 1992, 32 (01) :81-84
[7]  
GomezChiarri M, 1996, AM J PATHOL, V148, P301
[8]   IMMUNE-MECHANISMS IN ATHEROSCLEROSIS [J].
HANSSON, GK ;
JONASSON, L ;
SEIFERT, PS ;
STEMME, S .
ARTERIOSCLEROSIS, 1989, 9 (05) :567-578
[9]   INHIBITION OF CULTURED VASCULAR SMOOTH-MUSCLE CELL-MIGRATION BY SIMVASTATIN (MK-733) [J].
HIDAKA, Y ;
EDA, T ;
YONEMOTO, M ;
KAMEI, T .
ATHEROSCLEROSIS, 1992, 95 (01) :87-94
[10]   DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER-RNA BY MEANS OF THE POLYMERASE CHAIN-REACTION [J].
LIANG, P ;
PARDEE, AB .
SCIENCE, 1992, 257 (5072) :967-971