DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter

被引：16

作者：

Negre, D

BonodBidaud, C

Oudot, C

Prost, JF

Kolb, A

Ishihama, A

Cozzone, AJ

Cortay, JC

机构：

[1] INST BIOL & CHIM PROT,CNRS,F-69367 LYON,FRANCE

[2] INST PASTEUR,F-75724 PARIS,FRANCE

[3] NATL INST GENET,DEPT MOL GENET,MISHIMA,SHIZUOKA 411,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 04期

关键词：

D O I：

10.1093/nar/25.4.713

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The specific interaction of the upstream element-containing promoter of the Escherichia coli acetate operon with either the RNA polymerase holoenzyme or its alpha subunit has been analyzed by the base removal method, Our results indicate that: (i) direct and specific base contacts can be detected in the acetate promoter-alpha subunit complex; (ii) base elimination in the upstream element of the acetate promoter enhances the binding of RNA polymerase. A similar effect is observed when studying the interactions between RNA polymerase and the rrnB ribosomal operon P1 promoter.

引用

页码：713 / 718

页数：6

共 36 条

[1] DOMAIN ORGANIZATION OF RNA-POLYMERASE ALPHA-SUBUNIT - C-TERMINAL-85 AMINO-ACIDS CONSTITUTE A DOMAIN CAPABLE OF DIMERIZATION AND DNA-BINDING [J].