In vitro and in vivo anti-influenza A virus activity of antisense oligonucleotides

被引：7

作者：

Abe, T ^{[1
]}

Mizuta, T

Suzuki, S

Hatta, T

Takai, K

Yokota, T

Takaku, H

机构：

[1] Chiba Inst Technol, Dept Ind Chem, Chiba 2750016, Japan

[2] Rat Drug Design Labs, Fukushima 9601242, Japan

来源：

NUCLEOSIDES & NUCLEOTIDES | 1999年 / 18卷 / 6-7期

关键词：

D O I：

10.1080/07328319908044823

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have demonstrated that antisense phosphorothioate oligonucleotides (S-ODNs) inhibit influenza virus A replication in MDCK cells. The liposomally encapsulated and the free antisense phosphorothioate oligonucleotides with four target sites (PB1, PB2, PA, and NP) were tested for their abilities to inhibit virus-induced cytopathogenic effects by a MTT assay using MDCK cells. The liposomally encapsulated S-ODN complementary to the sites of the PB2-AUG initiation codon showed highly inhibitory effects. Therefore, the antiviral effects of S-ODN-PB2-AUG and PA-AUG were examined in a mouse model of influenza virus A infection. PB2-AUG oligomer treated i.v. significantly prolonged the mean survival time in day (MDS) and increased the survival rates with does dependent manner.

引用

页码：1685 / 1688

页数：4

共 3 条

[1] Specific inhibition of influenza virus RNA polymerase and nucleoprotein gene expression by liposomally encapsulated antisense phosphorothioate oligonucleotides in MDCK cells [J].