Knockdown of the novel proteasome subunit Adrm1 located on the 20q13 amplicon inhibits colorectal cancer cell migration, survival and tumorigenicity

被引：44

作者：

Chen, Wei ^{[1
]}

Hu, Xiao-Tong ^{[2
,3
]}

Shi, Qing-Lan ^{[2
,3
]}

Zhang, Fu-Biao ^{[2
,3
]}

He, Chao ^{[1
,2
,3
]}

机构：

[1] Zhejiang Univ, Dept Colorectal Surg, Sir Run Run Shaw Hosp, Hangzhou 310016, Zhejiang, Peoples R China

[2] Zhejiang Univ, Biomed Res Ctr, Sir Run Run Shaw Hosp, Hangzhou 310016, Zhejiang, Peoples R China

[3] Key Lab Biotherapy Zhejiang Prov, Hangzhou 310016, Zhejiang, Peoples R China

来源：

ONCOLOGY REPORTS | 2009年 / 21卷 / 02期

关键词：

adhesion-regulating molecule 1; shRNA; colorectal cancer; 20q13; amplicon; COMPARATIVE GENOMIC HYBRIDIZATION; DEUBIQUITINATING ENZYME; CHROMOSOMAL GAINS; UCH37; ADENOCARCINOMAS; IDENTIFICATION; CARCINOMA; PROTEIN; CLONING; TUMORS;

D O I：

10.3892/or_00000254

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The novel proteasome subunit Adrm1 located on the 20q13 amplicon was differentially expressed in colorectal cancer by semiquantitative RT-PCR. Adrm1 mRNA was overexpressed in 46.2% (18/39) colorectal cancer tissues compared to their matched normal mucosa and significantly correlated with lymph node metastasis of colorectal cancer (P=0.037). Knockdown of Adrm1 by shRNA in human colon carcinoma RKO cells inhibited their anchorage-independent growth, cell migration as well as cell proliferation through inducing apoptosis and cell cycle arrest at the G, phase. In addition, stable RNA interference of Adrm1 gene synergistic with 5-Fu treatment suppressed RKO cell growth in vitro. Collectively, these data suggested that Adrm1 is potentially oncogenic and may play an important role in colon tumorigenesis. Regiment with combined application of Adrm1 RNA interference and chemotherapy may emerge as a novel therapeutic strategy for Adrm1 overexpressed colorectal cancer.

引用

页码：531 / 537

页数：7

共 22 条

[1] BARDI G, 1993, CANCER, V71, P306, DOI 10.1002/1097-0142(19930115)71:2<306::AID-CNCR2820710207>3.0.CO
[2] 2-C
[3] KARYOTYPIC CHARACTERIZATION OF COLORECTAL ADENOCARCINOMAS
BARDI, G
SUKHIKH, T
PANDIS, N
FENGER, C
KRONBORG, O
HEIM, S
[J]. GENES CHROMOSOMES & CANCER, 1995, 12 (02) : 97 - 109
[4] A GENETIC MODEL FOR COLORECTAL TUMORIGENESIS
FEARON, ER
VOGELSTEIN, B
[J]. CELL, 1990, 61 (05) : 759 - 767
[5] FEJZO MS, GENES CHROM IN PRESS
[6] A novel proteasome interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes
Hamazaki, Jun
Iemura, Shun-ichiro
Natsume, Tohru
Yashiroda, Hideki
Tanaka, Keiji
Murata, Shigeo
[J]. EMBO JOURNAL, 2006, 25 (19) : 4524 - 4536
[7] Hu XT, 2008, ONCOL REP, V19, P441
[8] Adrm1, a putative cell adhesion regulating protein, is a novel proteasome-associated factor
Jorgensen, Jakob Ploug
Lauridsen, Anne-Marie
Kristensen, Poul
Dissing, Karen
Johnsen, Anders H.
Hendil, Klavs B.
Hartmann-Petersen, Rasmus
[J]. JOURNAL OF MOLECULAR BIOLOGY, 2006, 360 (05) : 1043 - 1052
[9] How proteolysis drives the cell cycle
King, RW
Deshaies, RJ
Peters, JM
Kirschner, MW
[J]. SCIENCE, 1996, 274 (5293) : 1652 - 1659
[10] Array CGH identifies distinct DNA copy number profiles of oncogenes and tumor suppressor genes in chromosomal- and microsatellite-unstable sporadic colorectal carcinomas
Lassmann, Silke
Weis, Roland
Makowiec, Frank
Roth, Jasmine
Danciu, Mihai
Hopt, Ulrich
Werner, Martin
[J]. JOURNAL OF MOLECULAR MEDICINE-JMM, 2007, 85 (03): : 289 - 300

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