Cloning and characterization of a novel human pantothenate kinase gene

被引：12

作者：

Ni, XH ^{[1
]}

Ma, YS ^{[1
]}

Cheng, HP ^{[1
]}

Jiang, M ^{[1
]}

Ying, K ^{[1
]}

Xie, Y ^{[1
]}

Mao, YM ^{[1
]}

机构：

[1] Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2002年 / 34卷 / 02期

关键词：

pantothenate kinase; northern blot; chromosome; 10q22.3-10q23.2;

D O I：

10.1016/S1357-2725(01)00114-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pantothenate kinase (PanK) is a key regulatory enzyme in the CoA biosynthetic pathway in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA. Here we report the molecular cloning and characterization of a new human PanK gene. The gene encoded a protein of 314 amino acid residues, which share high homology to mouse pantothenate kinase (mPanK) 1beta. Northern blot analysis revealed a transcript of the gene of 2.6 kb in human liver and kidney. The human PanK gene was located to human chromosome between 10q22.3 and 10q23.2 by bioinformatics analysis. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：109 / 115

页数：7

共 17 条

[1] Cloning and characterization of a eukaryotic pantothenate kinase gene (panK) from Aspergillus nidulans [J].

Calder, RB ;

Williams, RSB ;

Ramaswamy, G ;

Rock, CO ;

Campbell, E ;

Unkles, SE ;

Kinghorn, JR ;

Jackowski, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (04) :2014-2020

[2] COENZYME-A BIOSYNTHESIS IN PLANTS - PARTIAL-PURIFICATION AND CHARACTERIZATION OF PANTOTHENATE KINASE FROM SPINACH [J].

FALK, KL ;

GUERRA, DJ .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 301 (02) :424-430

[3] CLONING, SEQUENCE, AND EXPRESSION OF THE PANTOTHENATE PERMEASE (PANF) GENE OF ESCHERICHIA-COLI [J].

JACKOWSKI, S ;

ALIX, JH .

JOURNAL OF BACTERIOLOGY, 1990, 172 (07) :3842-3848

[4] PANTOTHENATE KINASE-ACTIVITY IN LIVERS OF GENETICALLY DIABETIC MICE (DB/DB) AND HORMONALLY TREATED CULTURED RAT HEPATOCYTES [J].

KIRSCHBAUM, N ;

CLEMONS, R ;

MARINO, KA ;

SHEEDY, G ;

NGUYEN, ML ;

SMITH, CM .

JOURNAL OF NUTRITION, 1990, 120 (11) :1376-1386

[5] THE EFFECT OF TUMOR-GROWTH ON LIVER PANTOTHENATE, COA, AND FATTY-ACID SYNTHETASE-ACTIVITY IN THE MOUSE [J].

MCALLISTER, RA ;

FIXTER, LM ;

CAMPBELL, EHG .

BRITISH JOURNAL OF CANCER, 1988, 57 (01) :83-86

[6] EFFECT OF L-CARNITINE ON CEREBRAL AND HEPATIC ENERGY METABOLITES IN CONGENITALLY HYPERAMMONEMIC SPARSE-FUR MICE AND ITS ROLE DURING BENZOATE THERAPY [J].

RATNAKUMARI, L ;

QURESHI, IA ;

BUTTERWORTH, RF .

METABOLISM-CLINICAL AND EXPERIMENTAL, 1993, 42 (08) :1039-1046

[7] Pantothenate kinase regulation of the intracellular concentration of coenzyme A [J].

Rock, CO ;

Calder, RB ;

Karim, MA ;

Jackowski, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (02) :1377-1383

[8] Transport and metabolism of the essential vitamin pantothenic acid in human erythrocytes infected with the malaria parasite Plasmodium falciparum [J].

Saliba, KJ ;

Horner, HA ;

Kirk, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (17) :10190-10195

[9]

SONG WJ, 1994, J BIOL CHEM, V269, P27051

[10] COAA AND RTS ARE ALLELIC AND LOCATED AT KILOBASE-3532 ON THE ESCHERICHIA-COLI PHYSICAL MAP [J].

SONG, WJ ;

JACKOWSKI, S .

JOURNAL OF BACTERIOLOGY, 1992, 174 (05) :1705-1706

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