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Sequence-specific interference by small RNAs derived from adenovirus VAI RNA

被引：84
作者：
Sano, M
Kato, Y
Taira, K
机构：
[1] AIST, Gene Funct Res Ctr, Tsukuba, Ibaraki 3058562, Japan
[2] Univ Tokyo, Sch Engn, Dept Chem & Biotechnol, Tokyo 1138656, Japan
来源：
FEBS LETTERS | 2006年 / 580卷 / 06期
关键词：
adenovirus; VAI RNA; dicer; siRNA; miRNA;
D O I：
10.1016/j.febslet.2006.01.085
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A virus-associated RNA (VAI) of adenoviruses is a cytoplasmic non-coding RNA and it plays an important role for viral replication in infected cells. VAI RNA transcripts, produced by RNA polymerase III (pol III), form tightly structured stems, which confer resistance to cellular defense systems. We demonstrate here that small RNAs of approximately 22 nucleotides are produced from a terminal stem region but not from an apical stem of VAI RNA. We determined the processing sites of VAI RNA by S1 nuclease mapping and further confirmed that the processed small RNA can act as small interfering RNAs (siRNAs) or as microRNAs (miRNAs) in transient transfection assays and during viral infection. Our data demonstrate that non-coding RNAs synthesized by pol III can be substrates for Dicer, and diced small RNAs might regulate cellular phenomena as siRNAs and miRNAs. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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页码：1553 / 1564
页数：12
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