Fluorescence micro-imaging of living cells and biomolecules with ultra high NA objectives

被引：6

作者：

Terakawa, S ^{[1
]}

Tsuboi, T ^{[1
]}

Sakurai, T ^{[1
]}

Jeromin, A ^{[1
]}

Wakazono, Y ^{[1
]}

Yamamoto, S ^{[1
]}

Abe, K ^{[1
]}

机构：

[1] Hamamatsu Univ Sch Med, Photon Med Res Ctr, Hamamatsu, Shizuoka 4313192, Japan

来源：

BIOPHOTONICS INSTRUMENTATION AND ANALYSIS | 2001年 / 4597卷

关键词：

total internal reflection flourescence microscopy(TIRFM); exocytosis; fluorescence resonance energy transfer;

D O I：

10.1117/12.446647

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Optical imaging with a high detecting power is very instrumental to dynamic observations of bio-molecular objects in water. To pioneer this field of imaging researches further, we used novel objective lenses of 1.65 and 1.45 in NA comparatively with 1.35 NA lens. The lenses of ultra high NA have a high resolving power and very useful properties for observation of high contrast images of fluorescently labeled molecules and subcellular organelles including DNA and membrane fusion proteins.

引用

页码：121 / 127

页数：7

共 7 条

[1] Selective imaging of surface fluorescence with very high aperture microscope objectives [J].

Axelrod, D .

JOURNAL OF BIOMEDICAL OPTICS, 2001, 6 (01) :6-13

[2] High numerical aperture objective lenses and optical system improved objective type total internal reflection fluorescence microscopy [J].

Kawano, Y ;

Abe, C ;

Kaneda, T ;

Aono, Y ;

Abe, K ;

Tamura, K ;

Terakawa, S .

OPTICAL DEVICES AND DIAGNOSTICS IN MATERIALS SCIENCE, 2000, 4098 :142-151

[3]

STAYER JA, 2001, NATURE REV, V2, P268

[4]

TERAKAWA S, 1997, BIOIMAGES, V5, P24

[5] Simultaneous evanescent wave imaging of insulin vesicle membrane and cargo during a single exocytotic event [J].

Tsuboi, T ;

Zhao, C ;

Terakawa, S ;

Rutter, GA .

CURRENT BIOLOGY, 2000, 10 (20) :1307-1310

[6] Protein kinase C-dependent supply of secretory granules to the plasma membrane [J].

Tsuboi, T ;

Kikuta, T ;

Warashina, A ;

Terakawa, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 282 (02) :621-628

[7] Transport, capture and exocytosis of single synaptic vesicles at active zones [J].

Zenisek, D ;

Steyer, JA ;

Almers, W .

NATURE, 2000, 406 (6798) :849-854

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