High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats

被引：353

作者：

Regina, A

Bird, A

Topping, D

Bowden, S

Freeman, J

Barsby, T

Kosar-Hashemi, B

Li, ZY

Rahman, S

Morell, M

机构：

[1] CSIRO, Food Futures Natl Res Flagship, N Ryde, NSW 1670, Australia

[2] CSIRO, Canberra, ACT 2601, Australia

[3] CSIRO, Adelaide, SA 5000, Australia

[4] Biogemma UK Ltd, Cambridge CB4 0GZ, England

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2006年 / 103卷 / 10期

关键词：

genetic engineering; nutrition; starch;

D O I：

10.1073/pnas.0510737103

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing > 70% amylose. When the > 70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.

引用

页码：3546 / 3551

页数：6

共 38 条

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