IL-1 beta induces the degradation of equine articular cartilage by a mechanism that is not mediated by nitric oxide

被引:33
作者
Bird, JLE [1 ]
Wells, T [1 ]
Platt, D [1 ]
Bayliss, MT [1 ]
机构
[1] UNIV LONDON ROYAL VET COLL,DEPT VET BASIC SCI,HATFIELD AL9 7TA,HERTS,ENGLAND
关键词
D O I
10.1006/bbrc.1997.7246
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Proteoglycan degradation was induced in young equine articular cartilage explants cultured for eight days in the presence of 50ng/ml recombinant human interleukin-1 beta. Degradation was initiated after 6 hours of exposure to the cytokine. This was accompanied by an induction of nitric oxide synthesis and a decrease in the incorporation of [S-35]sulphate into the glycosaminoglycan chains of proteoglycans. The addition of 1 mM N-iminoethyl-L-ornithine (an inhibitor of nitric oxide synthase) to the explant cultures in the presence of rhIL-1 beta suppressed the synthesis of NO and restored proteoglycan synthesis to control levels. However, treatment of explants with LNIO did not overcome proteoglycan degradation. These results indicate that although IL1 beta regulates both proteoglycan synthesis and degradation in equine cartilage explants, only the inhibition of proteoglycan synthesis is mediated by nitric oxide. (C) 1997 Academic Press.
引用
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页码:81 / 85
页数:5
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