A low-density DNA microarray for analysis of markers in breast cancer

被引:13
作者
Lacroix, M
Zammatteo, N
Remacle, J
Leclercq, G
机构
[1] Free Univ Brussels, Inst Jules Bordet, Lab Jean Claude Heuson Cancerol Mammaire, B-1000 Brussels, Belgium
[2] Fac Univ Notre Dame Paix, Lab Biochim & Biol Cellulaire, B-5000 Namur, Belgium
关键词
breast cancer; molecular markers; diagnosis; prognosis; prediction; microarray; chip; low-density; mRNA; DNA;
D O I
10.1177/172460080201700102
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Breast cancer remains a major cause of death in women from Western countries. In the near future, advances in both nucleic acids technology and tumor biology should be widely exploited to improve the diagnosis, prognosis, and outcome prediction of this disease. The DNA microarray, also called biochip, is a promising tool for performing massive, simultaneous, fast, and standardized analyses of multiple molecular markers in tumor samples. However, most currently available microarrays are expensive, which is mainly due to the amount (several thousands) of different DNA capture sequences that they carry. While these high-density microarrays are best suited for basic studies, their introduction into the clinical routine remains hypothetical. We describe here the principles of a low-density microarray, carrying only a few hundreds of capture sequences specific to markers whose importance in breast cancer is generally recognized or suggested by the current medical literature. We provide a list of about 250 of these markers. We also examine some potential difficulties (homologies between marker and/or variant sequences, size of sequences, etc.) associated with the production of such a low-cost microarray.
引用
收藏
页码:5 / 23
页数:19
相关论文
共 35 条
  • [1] Baruch A, 1999, CANCER RES, V59, P1552
  • [2] Validation of tissue microarray technology in breast carcinoma
    Camp, RL
    Charette, LA
    Rimm, DL
    [J]. LABORATORY INVESTIGATION, 2000, 80 (12) : 1943 - 1949
  • [3] Chappell SA, 2000, J PATHOL, V192, P159
  • [4] Regulation of keratin 19 gene expression by estrogen in human breast cancer cells and identification of the estrogen responsive gene region
    Choi, I
    Gudas, LJ
    Katzenellenbogen, BS
    [J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2000, 164 (1-2) : 225 - 237
  • [5] Cuny M, 2000, CANCER RES, V60, P1077
  • [6] Davies H, 1999, BIOTECHNIQUES, V27, P1258
  • [7] De Benedetti VMG, 2000, INT J BIOL MARKER, V15, P1
  • [8] A physical map of 30,000 human genes
    Deloukas, P
    Schuler, GD
    Gyapay, G
    Beasley, EM
    Soderlund, C
    Rodriguez-Tomé, P
    Hui, L
    Matise, TC
    McKusick, KB
    Beckmann, JS
    Bentolila, S
    Bihoreau, MT
    Birren, BB
    Browne, J
    Butler, A
    Castle, AB
    Chiannilkulchai, N
    Clee, C
    Day, PJR
    Dehejia, A
    Dibling, T
    Drouot, N
    Duprat, S
    Fizames, C
    Fox, S
    Gelling, S
    Green, L
    Harrison, P
    Hocking, R
    Holloway, E
    Hunt, S
    Keil, S
    Lijnzaad, P
    Louis-Dit-Sully, C
    Ma, J
    Mendis, A
    Miller, J
    Morissette, J
    Muselet, D
    Nusbaum, HC
    Peck, A
    Rozen, S
    Simon, D
    Slonim, DK
    Staples, R
    Stein, LD
    Stewart, EA
    Suchard, MA
    Thangarajah, T
    Vega-Czarny, N
    [J]. SCIENCE, 1998, 282 (5389) : 744 - 746
  • [9] Fitzgibbons PL, 2000, ARCH PATHOL LAB MED, V124, P966
  • [10] Foekens JA, 1999, INT J CANCER, V84, P209, DOI 10.1002/(SICI)1097-0215(19990621)84:3<209::AID-IJC2>3.3.CO