Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites

被引：327

作者：

Park, Jeongbin ^{[1
]}

Bae, Sangsu ^{[1
,2
]}

Kim, Jin-Soo ^{[3
,4
]}

机构：

[1] Hanyang Univ, Dept Chem, Seoul 133791, South Korea

[2] Hanyang Univ, Inst Mat Design, Seoul 133791, South Korea

[3] Inst for Basic Sci Korea, Ctr Genome Engn, Seoul, South Korea

[4] Seoul Natl Univ, Dept Chem, Seoul, South Korea

来源：

BIOINFORMATICS | 2015年 / 31卷 / 24期

关键词：

GUIDE; CRISPR/CAS9; DNA;

D O I：

10.1093/bioinformatics/btv537

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.

引用

页码：4014 / 4016

页数：3

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