Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor

被引：102

作者：

Thömmes, K ^{[1
]}

Lennartsson, J ^{[1
]}

Carlberg, M ^{[1
]}

Rönnstrand, L ^{[1
]}

机构：

[1] Ludwig Inst Canc Res, Biomedicum, S-75124 Uppsala, Sweden

来源：

BIOCHEMICAL JOURNAL | 1999年 / 341卷

关键词：

adapter protein; autophosphorylation; Ras MAP kinase pathway; SH2; domain; tyrosine phosphorylation;

D O I：

10.1042/0264-6021:3410211

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper we demonstrate the presence of two novel in vivo autophosphorylation sites in the c-Kit/stem cell factor receptor (c-Kit/SCFR): Tyr-703 in the kinase insert and Tyr-936 in the C-terminal tail. We furthermore demonstrate that the adapter protein Grb2 is a specific binding partner for both phosphorylated Tyr-703 and phosphorylated Tyr-936, whereas the adapter protein Grb7 binds selectively to phosphorylated Tyr-936. It is shown that the association occurs through the Src homology 2 (SH2) domains of Grb2 and Grb7. Binding of Grb2 to Tyr-703 in the c-Kit/SCFR provides a link to the Ras/mitogen-activated protein kinase pathway.

引用

页码：211 / 216

页数：6

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