Characterization of isolated ventricular myocytes from adult zebrafish (Danio rerio)

被引：83

作者：

Brette, Fabien ^{[1
]}

Luxan, Guillermo ^{[1
]}

Cros, Caroline ^{[1
]}

Dixey, Hayley ^{[1
]}

Wilson, Christopher ^{[1
]}

Shiels, Holly A. ^{[1
]}

机构：

[1] Univ Manchester, Fac Life Sci, Floor Core Technol Facil 2, Manchester M13 9NT, Lancs, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 374卷 / 01期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

heart; cardiac myocytes; ion channels; zebrafish; enzymatic isolation;

D O I：

10.1016/j.bbrc.2008.06.109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The zebrafish is widely used for human related disease studies. Surprisingly, there is no information about the electrical activity of single myocytes freshly isolated from adult zebrafish ventricle. In this Study, we present an enzymatic method to isolate ventricular myocytes from zebrafish heart that yield a large number of calcium tolerant cells. Ventricular myocytes from zebrafish were imaged using light and confocal microscopy. Myocytes were mostly rod shaped and responded by vigorous contraction to field electrical stimulation. Whole cell configuration of the patch clamp technique was used to record electrophysiological characteristics of myocytes. Action potentials present a long duration and a plateau phase and action potential duration decreases when increasing stimulation frequency (as observed in larger mammals). Together these results indicate that zebrafish is a species ideally Suited for investigation of ion channels related mutation Screening of cardiac alteration important in human. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：143 / 146

页数：4

共 18 条

[1] Zebrafish model for human long QT syndrome [J].