Improving DNA vaccine potency by linking Marck's disease virus type 1 VP22 to an antigen

被引：70

作者：

Hung, CF ^{[1
]}

He, LM ^{[1
]}

Juang, J ^{[1
]}

Lin, TJ ^{[1
]}

Ling, M ^{[1
]}

Wu, TC ^{[1
]}

机构：

[1] Johns Hopkins Med Inst, Baltimore, MD 21205 USA

来源：

JOURNAL OF VIROLOGY | 2002年 / 76卷 / 06期

关键词：

D O I：

10.1128/JVI.76.6.2676-2682.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have previously employed an intercellular spreading strategy using herpes simplex virus type I (HSV-1) VP22 protein to enhance DNA vaccine potency because DNA vaccines lack the intrinsic ability to amplify in cells. Recently, studies have demonstrated that the protein encoded by UL49 of Marek's disease virus type I (MDV-1) exhibits some degree of homology to the HSV-1 VP22 protein and features the property of intercellular transport. We therefore generated a DNA vaccine encoding MDV-1 1022 linked to a model antigen, human papillomavirus type 16 E7. We demonstrated that compared with mice vaccinated with DNA encoding wild-type E7, mice vaccinated with MDV-1 VP22/E7 DNA exhibited a significant increase in number of gamma-interferon-secreting, E7-specific CD8(+)-T-cell precursors as well as stronger tumor prevention and treatment effects. Furthermore, our data indicated that the antitumor effect was CD8 dependent. These results suggested that the development of vaccines encoding VP22 fused to a target antigen might be a promising strategy for improving DNA vaccine potency.

引用

页码：2676 / 2682

页数：7

共 37 条

[1] Mapping of herpes simplex virus-1 VP22 functional domains for inter- and subcellular protein targeting [J].