A scientific note on the detection of honeybee viruses using real-time PCR (TaqMan) in Varroa mites collected from a Thai honeybee (Apis mellifera) apiary

被引:122
作者
Chantawannakul, P [1 ]
Ward, L
Boonham, N
Brown, M
机构
[1] Chiang Mai Univ, Fac Sci, Dept Biol, Chiang Mai 50200, Thailand
[2] Cent Sci Lab, York YO41 1LZ, N Yorkshire, England
关键词
Varroa destructor; bee viruses; disease transmission; Apis mellifera;
D O I
10.1016/j.jip.2005.11.001
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Bee parasitic mite syndrome is a disease complex of colonies simultaneously infested with Varroa destrucior mites and infected with viruses and accompanied by high mortality. By using real-time PCR (TaqMan),. five out of seven bee viruses were detected in mite samples (V. destructor) collected from Thailand. Moreover, the results Of this Study provide an evidence for the co-existence of several bee viruses in a single mite. This is also the first report of bee viruses in mites from Thailand. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:69 / 73
页数:5
相关论文
共 15 条
[1]   The incidence and world distribution of honey bee viruses [J].
Allen, M ;
Ball, B .
BEE WORLD, 1996, 77 (03) :141-162
[2]   THE PREVALENCE OF PATHOGENS IN HONEY BEE (APIS-MELLIFERA) COLONIES INFESTED WITH THE PARASITIC MITE VARROA-JACOBSONI [J].
BALL, BV ;
ALLEN, MF .
ANNALS OF APPLIED BIOLOGY, 1988, 113 (02) :237-244
[3]   The detection of Tomato spotted wilt virus (TSWV) in individual thrips using real time fluorescent RT-PCR (TaqMan) [J].
Boonham, N ;
Smith, P ;
Walsh, K ;
Tame, J ;
Morris, J ;
Spence, N ;
Bennison, J ;
Barker, I .
JOURNAL OF VIROLOGICAL METHODS, 2002, 101 (1-2) :37-48
[4]  
BOVENWALKER PL, 1999, OUD J INVERTEBR PATH, V73, P101
[5]   Quantitative real-time reverse transcription-PCR analysis of deformed wing virus infection in the honeybee (Apis mellifera L.) [J].
Chen, YP ;
Higgins, JA ;
Feldlaufer, MF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (01) :436-441
[6]   Transmission of Kashmir bee virus by the ectoparasitic mite Varroa destructor [J].
Chen, YP ;
Pettis, JS ;
Evans, JD ;
Kramer, M ;
Feldlaufer, MF .
APIDOLOGIE, 2004, 35 (04) :441-448
[7]   The use of real-time RT-PCR (TaqMan®) and post-ELISA virus release for the detection of beet necrotic yellow vein virus types containing RNA 5 and its comparison with conventional RT-PCR [J].
Harju, VA ;
Skelton, A ;
Clover, GRG ;
Ratti, C ;
Boonham, N ;
Henry, CM ;
Mumford, RA .
JOURNAL OF VIROLOGICAL METHODS, 2005, 123 (01) :73-80
[8]   A scientific note on the detection of American strains of acute paralysis virus and Kashmir bee virus in dead bees in one US honey bee (Apis mellifera L) colony [J].
Hung, ACF ;
Ball, BV ;
Adams, JR ;
Shimanuki, H ;
Knox, DA .
APIDOLOGIE, 1996, 27 (01) :55-56
[9]   Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay [J].
Mumford, RA ;
Walsh, K ;
Barker, I ;
Boonham, N .
PHYTOPATHOLOGY, 2000, 90 (05) :448-453
[10]   Distribution of deformed wing virus within honey bee (Apis mellifera) brood cells infested with the ectoparasitic mite Varroa destructor [J].
Nordström, S .
EXPERIMENTAL AND APPLIED ACAROLOGY, 2003, 29 (3-4) :293-302