Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia

被引:38
作者
Ge, YM
Ezzell, RM
Clark, BD
Loiselle, PM
Amato, SF
Warren, HS
机构
[1] MASSACHUSETTS GEN HOSP, SHRINERS BURNS INST, INFECT DIS UNIT, CHARLESTOWN, MA 02129 USA
[2] MASSACHUSETTS GEN HOSP, SHRINERS BURNS INST, DEPT SURG, CHARLESTOWN, MA 02129 USA
[3] MASSACHUSETTS GEN HOSP, SHRINERS BURNS INST, DEPT MED, CHARLESTOWN, MA 02129 USA
[4] MASSACHUSETTS GEN HOSP, SHRINERS BURNS INST, DEPT PEDIAT, CHARLESTOWN, MA 02129 USA
[5] HARVARD UNIV, SCH MED, BOSTON, MA USA
[6] TUFTS UNIV, SCH MED, DEPT MED, DIV GEOG MED & INFECT DIS, BOSTON, MA 02111 USA
[7] TUFTS UNIV NEW ENGLAND MED CTR, BOSTON, MA 02111 USA
关键词
D O I
10.1086/514127
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Distributions of immunoreactive interleukin-l (IL-1) and lipopolysaccharide (LPS) were studied in the tissues of rats after intravenous injection of purified LPS or live Escherichia coli bacteria. IL-1 staining in the spleen peaked at 4-8 h, colocalized with LPS in marginal zone macrophages, and was undetectable 24 h after injection, whereas LPS staining peaked at 24 h and was detectable for 4 weeks, The tissue IL-1 response was similar for LPS and live bacteria. Thus, tissue IL-1 is downregulated within hours despite maintenance of LPS in the same cells for weeks, Macrophages in liver and lung had only slight IL-1 staining despite intense staining for LPS. Tissue IL-1 production appears to be differentially regulated after gram-negative bacteremia; LPS cleared by liver and lung macrophages elicit minimal IL-1, whereas there is high local IL-1 production in the marginal zone of the spleen that may increase immune responses to bacterial wall antigens.
引用
收藏
页码:1313 / 1321
页数:9
相关论文
共 43 条
[1]   PRODUCTION AND IN-VIVO USE OF RECOMBINANT OVINE IL-1-BETA AS AN IMMUNOLOGICAL ADJUVANT [J].
ANDREWS, AE ;
LOFTHOUSE, SA ;
BOWLES, VM ;
BRANDON, MR ;
NASH, AD .
VACCINE, 1994, 12 (01) :14-22
[3]  
BEUSCHER HU, 1987, J IMMUNOL, V139, P1896
[4]  
CANNON JG, 1989, J IMMUNOL, V142, P2299
[5]  
CHENSUE SW, 1991, AM J PATHOL, V138, P395
[6]   DETECTION OF INTERLEUKIN 1-ALPHA AND 1-BETA IN RABBIT-TISSUES DURING ENDOTOXEMIA USING SENSITIVE RADIOIMMUNOASSAYS [J].
CLARK, BD ;
BEDROSIAN, I ;
SCHINDLER, R ;
COMINELLI, F ;
CANNON, JG ;
SHAW, AR ;
DINARELLO, CA .
JOURNAL OF APPLIED PHYSIOLOGY, 1991, 71 (06) :2412-2418
[7]  
CONLON PJ, 1987, J IMMUNOL, V139, P98
[8]  
DIJKSTRA CD, 1985, IMMUNOLOGY, V54, P589
[9]   INTERLEUKIN-1 AND ITS BIOLOGICALLY RELATED CYTOKINES [J].
DINARELLO, CA .
ADVANCES IN IMMUNOLOGY, 1989, 44 :153-205
[10]   Biologic basis for interleukin-1 in disease [J].
Dinarello, CA .
BLOOD, 1996, 87 (06) :2095-2147