A high-performance neutron diffractometer for biological crystallography (BIX-3)

被引：71

作者：

Tanaka, I ^{[1
]}

Kurihara, K ^{[1
]}

Chatake, T ^{[1
]}

Niimura, N ^{[1
]}

机构：

[1] JAERI, Adv Sci Res Ctr, Tokai, Ibaraki 3191195, Japan

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2002年 / 35卷

关键词：

D O I：

10.1107/S0021889801017745

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A high-performance neutron diffractometer for biological crystallography (BIX-3) has been constructed at JRR-3M in the Japan Atomic Energy Research Institute (JAERI) in order to determine the hydrogen-atom positions in biological macromolecules. It uses several recent technical innovations, such as a neutron imaging plate and an elastically bent silicon monochromator developed by the authors. These have made it possible to realise a compact vertical arrangement of the diffractometer. Diffraction data have been collected from the proteins rubredoxin and myoglobin in about one month, to a resolution of 1.5 Angstrom. The data were good enough to identify the hydrogen atoms with high accuracy. By adopting a crystal-step scan method for measuring Bragg diffraction intensities, the signal-to-noise ratio was much better than that of the Laue method. This shows that BIX-3 is one of the best-performing machines for neutron protein crystallography in the world today.

引用

页码：34 / 40

页数：7

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