Hyperbranched Polyester Hydrogels with Controlled Drug Release and Cell Adhesion Properties

被引:110
作者
Zhang, Hongbin [1 ,2 ,3 ]
Patel, Alpesh [1 ,2 ]
Gaharwar, Akhilesh K. [4 ,5 ]
Mihaila, Silvia M. [1 ,2 ]
Iviglia, Giorgio [1 ,2 ]
Mukundan, Shilpaa [1 ,2 ]
Bae, Hojae [1 ,2 ]
Yang, Huai [3 ]
Khademhosseini, Ali [1 ,2 ,4 ]
机构
[1] Harvard Univ, Sch Med, Brigham & Womens Hosp, Ctr Biomed Engn,Dept Med, Cambridge, MA 02139 USA
[2] Harvard Mit Div Hlth Sci & Technol, MIT, Cambridge, MA 02139 USA
[3] Univ Sci & Technol Beijing, Beijing 100083, Peoples R China
[4] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA
[5] MIT, David H Koch Inst Integrat Canc Res, Cambridge, MA 02139 USA
基金
美国国家科学基金会;
关键词
POLY(ETHYLENE GLYCOL); MECHANICAL-PROPERTIES; OSTEOGENIC DIFFERENTIATION; TISSUE; DENDRIMERS; BIOMATERIALS; POLYMERS; SCAFFOLDS; DELIVERY; NANOCOMPOSITES;
D O I
10.1021/bm301825q
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Hyperbranched polyesters (HPE) have a high efficiency to encapsulate bioactive agents, including drugs, genes, and proteins, due to their globe-like nanostructure. However, the use of these highly branched polymeric systems for tissue engineering applications has not been broadly investigated. Here, we report synthesis and characterization of photocrosslinkable HPE hydrogels with sustained drug release characteristics for cellular therapies. These HPE can encapsulate hydrophobic drug molecules within the HPE cavities due to the presence of a hydrophobic inner structure that is otherwise difficult to achieve in conventional hydrogels. The functionalization of HPE with photocrosslinkable acrylate moieties renders the formation of hydrogels with a highly porous interconnected structure and mechanically tough network. The compressive modulus of HPE hydrogels was tunable by changing the crosslinking density. The feasibility of using these HPE networks for cellular therapies was investigated by evaluating cell adhesion, spreading, and proliferation on hydrogel surface. Highly crosslinked and mechanically stiff HPE hydrogels have higher cell adhesion, spreading, and proliferation compared to soft and complaint HPE hydrogels. Overall, we showed that hydrogels made from HPE could be used for biomedical applications that require spatial control of cell adhesion and controlled release of hydrophobic clues.
引用
收藏
页码:1299 / 1310
页数:12
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