Direct determination of the refractive index and thickness of a biolayer based on coupled waveguide-surface plasmon resonance mode

被引:34
作者
Chien, FC [1 ]
Chen, SJ
机构
[1] Natl Cent Univ, Inst Opt Sci, Chungli 320, Taiwan
[2] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 701, Taiwan
关键词
D O I
10.1364/OL.31.000187
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
A coupled waveguide-surface plasmon resonance (CWSPR) biosensor based on the Kretschmann configuration is developed. The CWSPR couples the surface plasmon resonance (SPR) mode and the waveguide mode and generates two sharp resonance dips in the reflectivity spectrum. The proposed biosensor not only retains the same sensing sensitivity as that of a conventional SPR device but also yields sharper dips in the reflectivity spectrum and therefore provides an improved measurement precision. The two reflectivity spectrum dips enable the refractive indices and thicknesses of both the self-assembled monolayer and a layer of human serum albumin absorbed dynamically on the sensing surface to be determined directly on a real-time basis. The CWSPR biosensor provides the capability to detect the biomolecular conformational changes that occur in biomolecular kinetic interactions. (c) 2006 Optical Society of America.
引用
收藏
页码:187 / 189
页数:3
相关论文
共 14 条