Ecdysteroid titers in mated and unmated Drosophila melanogaster females

被引：47

作者：

Harshman, LG ^{[1
]}

Loeb, AM ^{[1
]}

Johnson, BA ^{[1
]}

机构：

[1] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA

来源：

JOURNAL OF INSECT PHYSIOLOGY | 1999年 / 45卷 / 06期

关键词：

ecdysteroid titers; ecdysone; 20-hydroxyecdysone; Drosophila melanogaster;

D O I：

10.1016/S0022-1910(99)00038-4

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Radioimmunoassay was used to determine ecdysteroid titers in mated or unmated Drosophila melanogaster females. Whole-body ecdysteroid titers increase after mating and this response is more pronounced after 12-24 hours than it is immediately after mating. In one experiment, females were mated to transgenic males deficient in accessory gland proteins to test whether these peptides mediate the observed increase in female whole-body ecdysteroid titers. Females mated to such transgenic males do not show a pronounced increase in whole-body ecdysteroid titers. The effect of mating on female hemolymph ecdysteroid titers was also investigated. Hemolymph ecdysteroid titers decrease after mating. The ecdysteroid titer change in the hemolymph may result from yolk protein uptake of ecdysteroids into developing vitellogenic oocytes as a consequence of male accessory gland protein stimulation of female oocyte maturation and yolk protein synthesis following mating. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：571 / 577

页数：7

共 46 条

[1] ECTOPIC EXPRESSION OF SEX PEPTIDE ALTERS REPRODUCTIVE-BEHAVIOR OF FEMALE DROSOPHILA-MELANOGASTER [J].