Proteome analysis of hepatocellular carcinoma by laser capture microdissection

被引:57
作者
Ai, JH
Tan, YX
Ying, WT
Hong, Y
Liu, SQ
Wu, MC
Qian, XH
Wang, HY [1 ]
机构
[1] Second Mil Med Univ, Easter Hepatobiliary Surg Inst, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China
[2] Beijing Inst Radiat Med, Dept Genom & Prote, Beijing, Peoples R China
关键词
2-DE; hepatocellular carcinoma; laser capture microdissection;
D O I
10.1002/pmic.200500257
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Hepatocellular carcinoma (HCC) is one of the most frequent visceral neoplasia worldwide and is a multifactorial and multistage pathogenesis that finally leads to the deregulation of cell homeostasis. Laser capture microdissection (LCM) may allow a more ready identification of differences in protein expression in selected cell types or areas of tissue, and microscopic regions as small as 3-5 mu m in diameter can be sampled. Here we applied the LCM to the proteomic study of hepatitis B-related HCC and surrounding non-tumor tissues. Proteome alterations were observed using 2-DE and ESI-MS/MS, and alterations in the proteome were examined. Twenty protein spots were selected, of which 11 proteins were significantly altered in the HCC compared with the surrounding non-tumor tissues. Of the proteins that were selected, peroxiredoxin 2, apolipoprotein A-I precursor, 3-hydroxyacyl-CoA dehydrogenase type II, and 14.5-kDa translational inhibitor protein appear to be novel candidates as useful hepatitis B-related HCC markers. This study indicates that LCM is a useful technological method in the proteomic study of cancer tissue. The proteins revealed in this experiment can be used in the future for studies pertaining to hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HCC associated with hepatitis B virus infection.
引用
收藏
页码:538 / 546
页数:9
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