Selective loading of kinesin-powered molecular shuttles with protein cargo and its application to biosensing

被引：108

作者：

Ramachandran, S

Ernst, KH

Bachand, GD

Vogel, V

Hess, H ^{[1
]}

机构：

[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA

[2] Swiss Fed Inst Mat Sci & Technol EMPA, Mol Surface Sci Grp, Dubendorf, Switzerland

[3] Sandia Natl Labs, Biomol Mat & Interfaces Dept, Albuquerque, NM 87185 USA

[4] Sandia Natl Labs, Ctr Integrated Nanotechnol, Albuquerque, NM 87185 USA

[5] ETH Honggerberg, Dept Mat, CH-8093 Zurich, Switzerland

[6] Univ Florida, Dept Mat Sci & Engn, Gainesville, FL 32611 USA

来源：

SMALL | 2006年 / 2卷 / 03期

关键词：

biomolecular motors; biosensors; kinesin; microtubules; molecular shuttles;

D O I：

10.1002/smll.200500265

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The selective loading of Kinsein-powered molecular shuttles with protein cargo and its application in biosensing is discussed. The initial coating of the Kinsein-bound, biotinylated microtubules with streptavidin has to fill the available biotin binding sites to avoid unwanted cross-linking of microtubules with each other. It permits the selective capture of proteins, and the subsequent detection of the capture event in double-antibody sandwich geometry. It enables the loading of all cargo types against which antibodies can be raised.

引用

页码：330 / 334

页数：5

共 24 条

[1] Assembly and transport of nanocrystal CdSe quantum dot nanocomposites using microtubules and kinesin motor proteins [J].