Expression of interleukin-6 receptor (IL-6R) and gp130 mRNA in PC12 cells and sympathetic neurons: Modulation by tumor necrosis factor alpha (TNF-alpha)

被引:57
作者
Marz, P
Gadient, RA
Otten, U
机构
[1] UNIV BASEL, DEPT PHYSIOL, CH-4051 BASEL, SWITZERLAND
[2] CALTECH, DIV BIOL, PASADENA, CA 91125 USA
关键词
interleukin-6; receptor; Gp; 130; c-fos; reverse transcriptase-polymerase chain reaction; hybridization; in situ; PC12; cell; sympathetic neuron; tumor necrosis factor alpha; glucocorticoid; differentiation;
D O I
10.1016/0006-8993(95)01210-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Recent findings indicate that IL-6, besides its various biological effects, also exerts neurotrophic and neuroprotective functions. Using the pheochromocytoma cell line PC12 and cultured primary sympathetic neurons, we investigated whether neurons express the IL-6 receptors, IL-6R and gp130, and how they might be regulated. For these studies we used RT-PCR and in situ hybridization. We provide here evidence for the expression of functional IL-6Rs in peripheral sympathetic neurons and PC12 cells. Furthermore we demonstrate that cytokines modulate the expression of IL-BR and gp130 mRNA. This modulation is much more pronounced in neuronally-differentiated PC12 cells than in undifferentiated cells. Among various cytokines tested, tumor necrosis factor alpha (TNF-alpha) turned out to be a major regulator of the IL-6R and gp130 mRNA expression. The induction was time- and dose-dependent for both genes. Maximal induction was reached within 16 h at a concentration of 0.1 nM TNF-alpha. The stimulatory effect of TNF-alpha on the IL-6R system was completely inhibited by the simultaneous addition of the glucocorticoid dexamethasone. In summary, our results show that sympathetic neurons and neuron-like differentiated PC12 cells express functional IL-6R and gp130, and that the expression of their mRNAs is modulated by cytokines. We suggest that cytokines such as IL-6 can modulate sympathetic neuron function.
引用
收藏
页码:71 / 79
页数:9
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