White shrimp (Litopenaeus vannamei) recombinant lysozyme has antibacterial activity against Gram negative bacteria:: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholerae

被引：77

作者：

de-la-Re-Vega, E

García-Galaz, A

Díaz-Cinco, ME

Sotelo-Mundo, RR

机构：

[1] Ctr Invest Alimentac & Desarroloo, AC, Aquat Mol Biol Lab, Hermosillo 83000, Sonora, Mexico

[2] Ctr Invest Alimentac & Desarroloo, AC, Microbiol Lab, Hermosillo 83000, Sonora, Mexico

来源：

FISH & SHELLFISH IMMUNOLOGY | 2006年 / 20卷 / 03期

关键词：

lysozyme; recombinant protein; antibacterial; Gram negative; Vibrio alginolyticus; Vibrio parahemolyticus; Vibrio cholerae;

D O I：

10.1016/j.fsi.2005.06.005

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

C-type lysozyme has been described as an antibacterial component of the shrimp innate defence system. We determined quantitatively the antibacterial activity of white shrimp (Litopenaeus vannamei) recombinant lysozyme against three Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholerae, using a turbidimetric assay with live bacteria and differential bacterial viable count after interaction with the protein. In conclusion, the antibacterial activity of recombinant shrimp lysozyme against Vibrio sp. is at least equal to the values against the Gram positive Micrococcus luteus and more active against the shrimp pathogens V. alginolyticus and V. parahemolyticus. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：405 / 408

页数：4

共 12 条

[1]

GARCIAOROZCO KD, IN PRESS BIOTECH LET

[2]

Goarant C, 1999, APPL ENVIRON MICROB, V65, P1145

[3] Characterization and function of kuruma shrimp lysozyme possessing lytic activity against Vibrio species [J].