Candida albicans adhesion to human epithelial cells and polystyrene and formation of biofilm is reduced by sub-inhibitory Melaleuca alternifolia (tea tree) essential oil

被引:36
作者
Sudjana, Aurelia N. [1 ]
Carson, Christine F. [1 ]
Carson, Kerry C. [2 ]
Riley, Thomas V. [1 ,2 ]
Hammer, Katherine A. [1 ]
机构
[1] Univ Western Australia, Discipline Microbiol & Immunol M502, Sch Biomed Biomol & Chem Sci, Crawley, WA 6009, Australia
[2] PathWest Lab Med WA, Div Microbiol & Infect Dis, Nedlands, WA, Australia
关键词
candidiasis; virulence; alternative therapies; monoterpenes; IN-VITRO ACTIVITY; STAPHYLOCOCCUS-AUREUS; MECHANISMS; RESISTANT;
D O I
10.3109/13693786.2012.683540
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
This study investigated the effects of the volatile terpene-rich oil from Melaleuca alternifolia (tea tree oil) on the formation of biofilms and the adhesion of C. albicans cells to both biotic and abiotic surfaces. Biofilm formation on polystyrene was significantly inhibited for 70% of the isolates at the lowest test concentration of 0.016% of tea tree oil (TTO) when quantifi ed by XTT and 40% of isolates when measured by crystal violet staining. Adhesion to polystyrene, quantifi ed by crystal violet staining, was significantly reduced for 3 isolates at 0.031%, 6 isolates at 0.062% and 0.125% and for all 7 isolates at 0.25% TTO. Reductions in adhesion were not due to loss of viability (at concentrations of <= 0.125%) or interactions between the TTO and polystyrene. Similarly, adhesion to buccal epithelial and HeLa cells was also significantly reduced in the presence of 0.016-0.062% TTO. Treatment with 0.125% TTO, but not 0.062%, decreased the cell surface hydrophobicity of C. albicans, indicating one potential mechanism by which adhesion may be reduced. These data demonstrate that sub-inhibitory TTO reduces the adhesion of C. albicans to both human cells and polystyrene, inhibits biofilm formation and decreases cell surface hydrophobicity.
引用
收藏
页码:863 / 870
页数:8
相关论文
共 28 条
[1]   COMPARISON OF TRYPAN BLUE-DYE EXCLUSION AND FLUOROMETRIC ASSAYS FOR MAMMALIAN-CELL VIABILITY DETERMINATIONS [J].
ALTMAN, SA ;
RANDERS, L ;
RAO, G .
BIOTECHNOLOGY PROGRESS, 1993, 9 (06) :671-674
[2]  
[Anonymous], 2008, Approved standard M27-A. A National Committee for Clinical Laboratory Standards
[3]   In vitro activity of tea-tree oil against clinical skin isolates of meticillin-resistant and -sensitive Staphylococcus aureus and coagulase-negative staphylococci growing planktonically and as biofilms [J].
Brady, Aaron ;
Loughlin, Ryan ;
Gilpin, Deirdre ;
Kearney, Paddy ;
Tunney, Michael .
JOURNAL OF MEDICAL MICROBIOLOGY, 2006, 55 (10) :1375-1380
[4]   Thymol inhibits Candida albicans biofilm formation and mature biofilm [J].
Braga, Pier Carlo ;
Culici, Maria ;
Alfieri, Marina ;
Dal Sasso, Monica .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2008, 31 (05) :472-477
[5]   Melaleuca alternifolia (tea tree) oil:: a review of antimicrobial and other medicinal properties [J].
Carson, CF ;
Hammer, KA ;
Riley, TV .
CLINICAL MICROBIOLOGY REVIEWS, 2006, 19 (01) :50-+
[6]   Targeting virulence: a new paradigm for antimicrobial therapy [J].
Clatworthy, Anne E. ;
Pierson, Emily ;
Hung, Deborah T. .
NATURE CHEMICAL BIOLOGY, 2007, 3 (09) :541-548
[7]  
Colling L, 2000, INFECT DIS OBSTET GY, V13, P43
[8]  
Cotter G, 2000, BRIT J BIOMED SCI, V57, P241
[9]   In vitro activity of terpenes against Candida biofilms [J].
Dalleau, Stephanie ;
Cateau, Estelle ;
Berges, Thierry ;
Berjeaud, Jean-Marc ;
Imbert, Christine .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2008, 31 (06) :572-576
[10]   Characterization of Mucosal Candida albicans Biofilms [J].
Dongari-Bagtzoglou, Anna ;
Kashleva, Helena ;
Dwivedi, Prabhat ;
Diaz, Patricia ;
Vasilakos, John .
PLOS ONE, 2009, 4 (11)