Identification of molecular markers for the early detection of human squamous cell carcinoma of the uterine cervix

被引:34
作者
Cheng, Q
Lau, WM
Chew, SH
Ho, TH
Tay, SK
Hui, KM
机构
[1] Natl Canc Ctr, Gene Struct & Express Lab, Div Cellular & Mol Res, Singapore 169610, Singapore
[2] KK Womens & Childrens Hosp, Dept Pathol & Gynaecol Oncol, Singapore 229899, Singapore
[3] KK Womens & Childrens Hosp, Dept Gynaecol Oncol, Singapore 229899, Singapore
[4] Singapore Gen Hosp, Dept Obstet & Gynaecol, Singapore 169608, Singapore
基金
英国医学研究理事会;
关键词
human squamous cervical cancer; FIGO stages; gene expression; ribosomal protein S12; molecular diagnostic marker;
D O I
10.1038/sj.bjc.6600038
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To identify novel cellular genes that could potentially act as predictive molecular markers for human cervical cancer, we employed RT-PCR differential display, reverse Northern and Northern blot analysis to compare the gene expression profiles between squamous cell carcinoma biopsies and adjacent histo-pathological normal epithelium tissues, Twenty-eight cDNA clones were isolated that were demonstrated to be consistently over-expressed in squamous cell cervical cancer biopsies of FIGO stages 1B to 3B. Most importantly, it was observed that, in addition to their over-expression in cancer lesions, some of these genes are upregulated in the presumably histo-pathological normal adjacent tissues. Of particular interest is clone G30CC that has been identified to be the gene that encodes S 12 ribosomal protein. When employed for RNA - RNA in situ hybridization experiments, expression of G30CC could be detected in the immature basal epithelial cells of histo-pathological normal tissues collected from cervical cancer patients of early FIGO stages. In comparison, the expression of G30CC was not detected in cervical tissues collected from patients admitted for surgery of non-malignant conditions. These results allow the distinct possibility of employing the ribosomal protein S 12 gene as an early molecular diagnostic identifier for the screening of human cervical cancer and a potential target employed for cancer gene therapy trials. (C) 2002 The Cancer Research Campaign.
引用
收藏
页码:274 / 281
页数:8
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