Electrochemical study on the behavior of Morin and its interaction with DNA

Voltarnmetric behavior of Morin was studied in 0.1 M HAc-NaAc + 50 mM KCl (pH 3.4) solution at glassy carbon electrode (GCE) using cyclic voltammetry (CV). Morin showed an irreversible anodic peak at 0.720 V in CV which was involving two electrons and two protons. Also, the interaction of Morin with double-stranded calf thymus DNA (ctDNA) was studied by CV at GCE with an irreversible electrochemical equation. As a result of reaction with ctDNA, the voltammetric peak of Morin was a position shift and the peak current decreased. The diffusion coefficients of both free and binding Morin (D-f = 1. 1086 x 10(-7) cm(2).s(-1) and D-b = 8.2544 x 10(-9) cm(2) s(-1)), binding constant (K= 1.7765 x 10(7) cm(3) Mol(-1)), and binding site size (s = 0.8510) of the Morin-DNA complex were obtained simultaneously by non-linear fit analysis. The results demonstrate that Morin can bind to ctDNA in 0.1 M HAc-NaAc + 50 mM KCl (pH 3.4) solution and the ting B of Morin intercalates between the DNA base pairs. (c) 2005 Elsevier B.V. All rights reserved.