Crystallization and preliminary X-ray studies of metallothionein II from rabbit liver

被引：6

作者：

An, Y ^{[1
]}

Li, GP

Ru, BG

机构：

[1] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China

[2] Peking Univ, Dept Chem, Beijing 100871, Peoples R China

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444999005430

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Metallothionein II (M-w =6.8 kDa), induced by cadmium and purified from rabbit liver, has been crystallized in space group P6(2)22 or P6(4)22; The unit-cell parameters were a = b = 113.4, c = 219.1 Angstrom, alpha = beta = 90.0, gamma = 120.0 degrees when crystallized from sodium citrate buffer and a = b = 113.4, c = 219.6 Angstrom, alpha = beta = 90.0, gamma = 120.0 degrees when crystallized from Tris- HCl buffer. There are 12 molecules per asymmetric unit and the solvent: content is about 57%.

引用

页码：1242 / 1243

页数：2

共 6 条

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[3]

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[4] REFINED CRYSTAL-STRUCTURE OF CD, ZN METALLOTHIONEIN AT 2.0 A RESOLUTION [J].

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COLLETT, SA ;

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FUREY, WF ;

WANG, BC ;

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[5]

VALLEE BL, 1991, METHOD ENZYMOL, V205, P3

[6]

WESTBROOK EM, 1985, METHOD ENZYMOL, V114, P187

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