Adsorption of peptide nucleic acid and DNA decamers at electrically charged surfaces

被引:53
作者
Fojta, M
Vetterl, V
Tomschik, M
Jelen, F
Nielsen, P
Wang, J
Palecek, E
机构
[1] ACAD SCI CZECH REPUBL, INST BIOPHYS, CS-61265 BRNO, CZECH REPUBLIC
[2] IMBG, CTR BIOMOL RECOGNIT, DEPT BIOCHEM B, PANUM INST, DK-2200 COPENHAGEN, DENMARK
[3] NEW MEXICO STATE UNIV, DEPT CHEM & BIOCHEM, LAS CRUCES, NM 88003 USA
[4] MASARYK UNIV, FAC SCI, DEPT PHYS ELECT, CS-61137 BRNO, CZECH REPUBLIC
关键词
D O I
10.1016/S0006-3495(97)78873-8
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Adsorption behavior of peptide nucleic acid (PNA) and DNA decamers (GTAGATCACT and the complementary sequence) on a mercury surface was studied by means of AC impedance measurements at a hanging mercury drop electrode. The nucleic acid was first attached to the electrode by adsorption from a 5-mu l drop of PNA (or DNA) solution, and the electrode with the adsorbed nucleic acid layer was then washed and immersed in the blank background electrolyte where the differential capacity C of the electrodedouble layer was measured as a function of the applied potential E. It was found that the adsorption behavior of the PNA with an electrically neutral backbone differs greatly from that of the DNA (with a negatively charged backbone), whereas the DNA-PNA hybrid shows intermediate behavior. At higher surface coverage PNA molecules associate at the surface, and the minimum value of C is shifted to negative potentials because of intermolecular interactions of PNA at the surface, Prolonged exposure of PNA to highly negative potentials does not result in PNA desorption, whereas almost all of the DNA is removed from the surface at these potentials. Adsorption of PNA decreases with increasing NaCl concentration in the range from 0 to 50 mM NaCl, in contrast to DNA, the adsorption of which increases under the same conditions.
引用
收藏
页码:2285 / 2293
页数:9
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