Fabrication of 3-Dimensional Cellular Constructs via Microstereolithography Using a Simple, Three-Component, Poly(Ethylene Glycol) Acrylate-Based System

被引：24

作者：

Leigh, Simon J. ^{[2
]}

Gilbert, Hamish T. J. ^{[3
]}

Barker, Ian A. ^{[1
]}

Becker, Jan M. ^{[1
]}

Richardson, Stephen M. ^{[3
]}

Hoyland, Judith A. ^{[3
]}

Covington, James A. ^{[2
]}

Dove, Andrew P. ^{[1
]}

机构：

[1] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

[2] Univ Warwick, Sch Engn, Coventry CV4 7AL, W Midlands, England

[3] Univ Manchester, Fac Med & Human Sci, Inst Inflammat & Repair, Manchester M13 9PT, Lancs, England

来源：

BIOMACROMOLECULES | 2013年 / 14卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

MESENCHYMAL STEM-CELLS; POLYMERIZATION SHRINKAGE; MICRO-STEREOLITHOGRAPHY; DIFFERENTIATION; SCAFFOLDS; HYDROGELS; DESIGN; MICROFABRICATION; MODULATION; ADHESION;

D O I：

10.1021/bm3015736

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

A novel method for the production of inhibitor-and solvent-free resins suitable for three-dimensional (3D) microstereolithography is reported. Using an exemplar poly(ethylene glycol) based resin, the control of features in the X, Y, and Z planes is demonstrated such that complex structures can be manufactured Human mesenchymal stem cells cultured on the manufactured scaffolds remained viable during the 7 day assessment period, with proliferation rates comparable to those observed on tissue culture polystyrene. These data suggest that this novel, yet simple, method is suitable for production of 3D scaffolds for tissue engineering and regenerative medicine applications.

引用

页码：186 / 192

页数：7

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