Evaluating the potential of SSR: flanking regions for examining taxonomic relationships in the Vitaceae

被引：103

作者：

Rossetto, M ^{[1
]}

McNally, J ^{[1
]}

Henry, RJ ^{[1
]}

机构：

[1] So Cross Univ, Ctr Plant Conservat Genet, Lismore, NSW 2480, Australia

来源：

THEORETICAL AND APPLIED GENETICS | 2002年 / 104卷 / 01期

关键词：

microsatellites; Vitis; mutation; flanking regions; ESTs;

D O I：

10.1007/s001220200007

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Three EST-derived microsatellite loci from Vitis vinifera were amplified and sequenced across eight species of Vitaceae from four different genera. Phylogenetic analysis of the microsatellite's flanking regions produced informative results in congruence with previous studies. Generic relationships were respected and the data produced sufficient inter-specific variation to distinguish between Cayratia acris and Cayratia saponaria, two very closely related species. Overall, the sequence alignments showed that priming sites were conserved, whereas microsatellite repeats were present in most cases but structurally variable. The sequence data provided information on the evolutionary patterns of various microsatellite repeats and their correlation to evolutionary relationships among taxa.

引用

页码：61 / 66

页数：6

共 27 条

[1]

[Anonymous], 2000, Phylogenetic analysis using parsimony (*and other methods)

[2]

Bentham G, 1863, FLORA AUSTRALIENSIS, V1

[3] Optimisation of a microsatellite enrichment technique in Saccharum spp. [J].

Cordeiro, GM ;

Maguire, TL ;

Edwards, KJ ;

Henry, RJ .

PLANT MOLECULAR BIOLOGY REPORTER, 1999, 17 (03) :225-229

[4] MUTATIONAL PROCESSES OF SIMPLE-SEQUENCE REPEAT LOCI IN HUMAN-POPULATIONS [J].

DIRIENZO, A ;

PETERSON, AC ;

GARZA, JC ;

VALDES, AM ;

SLATKIN, M ;

FREIMER, NB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :3166-3170

[5] Size homoplasy in chloroplast microsatellites of wild perennial relatives of soybean (Glycine subgenus Glycine) [J].

Doyle, JJ ;

Morgante, M ;

Tingey, SV ;

Powell, W .

MOLECULAR BIOLOGY AND EVOLUTION, 1998, 15 (02) :215-218

[6]

EDWARDS KJ, 1996, BIOTECHNIQUES, V20, P759

[7]

ESTOUP A, 1995, GENETICS, V140, P679

[8]

FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x

[9] TOWARD DEFINING COURSE OF EVOLUTION - MINIMUM CHANGE FOR A SPECIFIC TREE TOPOLOGY [J].

FITCH, WM .

SYSTEMATIC ZOOLOGY, 1971, 20 (04) :406-&

[10]

GARZA JC, 1995, MOL BIOL EVOL, V12, P594

← 1 2 3 →