Alternative donor substrates for inverting and retaining glycosyltransferases

被引：31

作者：

Lairson, Luke L.

Wakarchuk, Warren W.

Withers, Stephen G. ^{[1
]}

机构：

[1] Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z1, Canada

[2] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

[3] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V6T 1Z3, Canada

来源：

CHEMICAL COMMUNICATIONS | 2007年 / 04期

关键词：

D O I：

10.1039/b614636h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

By the use of glycosyl donors containing aromatic leaving groups linked with opposite anomeric configurations compared to those of the natural donor substrates, an inverting (Cst II) and a retaining (LgtC) glycosyltransferase were found to catalyse glycosylation reactions of natural acceptor substrates in the presence of the corresponding nucleotide.

引用

收藏

页码：365 / 367

页数：3

相关论文

共 22 条

[1] Searching for medicine's sweet spot [J].

Alper, J .

SCIENCE, 2001, 291 (5512) :2338-2343

[2] Chemical glycobiology [J].

Bertozzi, CR ;

Kiessling, LL .

SCIENCE, 2001, 291 (5512) :2357-2364

[3] Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog [J].

Chiu, CPC ;

Watts, AG ;

Lairson, LL ;

Gilbert, M ;

Lim, D ;

Wakarchuk, WW ;

Withers, SG ;

Strynadka, NCJ .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (02) :163-170

[4] An evolving hierarchical family classification for glycosyltransferases [J].

Coutinho, PM ;

Deleury, E ;

Davies, GJ ;

Henrissat, B .

JOURNAL OF MOLECULAR BIOLOGY, 2003, 328 (02) :307-317

[5] Bovine α1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases [J].

Gastinel, LN ;

Bignon, C ;

Misra, AK ;

Hindsgaul, O ;

Shaper, JH ;

Joziasse, DH .

EMBO JOURNAL, 2001, 20 (04) :638-649

[6] The synthesis of sialylated oligosaccharides using a CMP-Neu5Ac synthetase/sialyltransferase fusion [J].

Gilbert, M ;

Bayer, R ;

Cunningham, AM ;

Defrees, S ;

Gao, YH ;

Watson, DC ;

Young, NM ;

Wakarchuk, WW .

NATURE BIOTECHNOLOGY, 1998, 16 (08) :769-772

[7] DEFINING THE MINIMAL SIZE OF CATALYTICALLY ACTIVE PRIMATE ALPHA-1,3 GALACTOSYLTRANSFERASE - STRUCTURE-FUNCTION STUDIES ON THE RECOMBINANT TRUNCATED ENZYME [J].

HENION, TR ;

MACHER, BA ;

ANARAKI, F ;

GALILI, U .

GLYCOBIOLOGY, 1994, 4 (02) :193-201

[8] Intermediate trapping on a mutant retaining α-galactosyltransferase identifies an unexpected aspartate residue [J].

Lairson, LL ;

Chiu, CPC ;

Ly, HD ;

He, SM ;

Wakarchuk, WW ;

Strynadka, NCJ ;

Withers, SG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (27) :28339-28344

[9] Mechanistic analogies amongst carbohydrate modifying enzymes [J].

Lairson, LL ;

Withers, SG .

CHEMICAL COMMUNICATIONS, 2004, (20) :2243-2248

[10] Glycosyl fluorides can function as substrates for nucleotide phosphosugar-dependent glycosyltransferases [J].

Lougheed, B ;

Ly, HD ;

Wakarchuk, WW ;

Withers, SG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (53) :37717-37722