Molecular typing of Yersinia frederiksenii strains by means of 16s rDNA and gyrB genes sequence analyses

被引：23

作者：

Demarta, A ^{[1
]}

De Respinis, S ^{[1
]}

Dolina, M ^{[1
]}

Peduzzi, R ^{[1
]}

机构：

[1] Ist Cantonale Microbiol, CH-6500 Bellinzona, Switzerland

来源：

FEMS MICROBIOLOGY LETTERS | 2004年 / 238卷 / 02期

关键词：

16S rDNA sequences; gyrB sequences; molecular typing; phylogenetic identification; genospecies; Yersinia frederiksenii;

D O I：

10.1016/j.femsle.2004.08.005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The phenospecies Yersinia frederiksenii is known to comprise three genospecies, indistinguishable on the basis of phenotypic characteristics. In previous works.. 13 strains, identified biochemically as Y. frederiksenii, were characterized using Multilocus enzyme electrophoresis and Ribotyping. In order to elucidate the phylogenetic position of these strains we performed their molecular typing by means of 16S rDNA and gyrB sequences analyses. Results demonstrated that gyrB is a better phylogenctic marker than 16S rDNA. The classification achieved by gyrB sequences analyses was in agreement with results obtained with more laborious methods. Moreover, a good phylogenctic identification could be reached also with partial gyrB sequences of only 350 bp. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：423 / 428

页数：6

共 15 条

[1]

Ausubel FA, 1995, CURRENT PROTOCOLS MO

[2] CHARACTERIZATION OF YERSINIA-ENTEROCOLITICA AND YERSINIA-PSEUDOTUBERCULOSIS BY DEOXYRIBONUCLEIC-ACID HYBRIDIZATION AND BY BIOCHEMICAL REACTIONS [J].

BRENNER, DJ ;

STEIGERWALT, AG ;

FALCAO, DP ;

WEAVER, RE ;

FANNING, GR .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1976, 26 (02) :180-194

[3] CONSTRUCTION AND FINE MAPPING OF RECOMBINANT PLASMIDS CONTAINING THE RRNB RIBOSOMAL-RNA OPERON OF ESCHERICHIA-COLI [J].

BROSIUS, J ;

ULLRICH, A ;

RAKER, MA ;

GRAY, A ;

DULL, TJ ;

GUTELL, RR ;

NOLLER, HF .

PLASMID, 1981, 6 (01) :112-118

[4] Sequence heterogeneities among 16S ribosomal RNA sequences, and their effect on phylogenetic analyses at the species level [J].

Cilia, V ;

Lafay, B ;

Christen, R .

MOLECULAR BIOLOGY AND EVOLUTION, 1996, 13 (03) :451-461

[5] Signature region within the 16S rDNA sequences of Aeromonas popoffii [J].

Demarta, A ;

Tonolla, M ;

Caminada, AP ;

Ruggeri, N ;

Peduzzi, R .

FEMS MICROBIOLOGY LETTERS, 1999, 172 (02) :239-246

[6] POPULATION-GENETICS OF HUMAN, ANIMAL, AND ENVIRONMENTAL YERSINIA STRAINS [J].

DOLINA, M ;

PEDUZZI, R .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (02) :442-450

[7]

DOLINA M, 1995, YERSINIOSIS PRESENT, V13, P140

[8] THE PHYLOGENY OF THE GENUS YERSINIA BASED ON 16S RDNA SEQUENCES [J].

IBRAHIM, A ;

GOEBEL, BM ;

LIESACK, W ;

GRIFFITHS, M ;

STACKEBRANDT, E .

FEMS MICROBIOLOGY LETTERS, 1993, 114 (02) :173-178

[9]

Ibrahim A, 1997, FEMS MICROBIOL LETT, V146, P73, DOI [10.1016/S0378-1097(96)00456-9, 10.1111/j.1574-6968.1997.tb10173.x]

[10] MEGA2: molecular evolutionary genetics analysis software [J].

Kumar, S ;

Tamura, K ;

Jakobsen, IB ;

Nei, M .

BIOINFORMATICS, 2001, 17 (12) :1244-1245

← 1 2 →