The effects of duodenal peptides on glucagon-like peptide-1 secretion from the ileum: A duodeno-ileal loop?

被引:57
作者
Hansen, L [1 ]
Holst, JJ [1 ]
机构
[1] Univ Copenhagen, Panum Inst, Dept Med Physiol, DK-2200 Copenhagen N, Denmark
关键词
isolated perfused porcine ileum; somatostatin; proglucagon; GIP; gastric inhibitory polypeptide; CCK; 8; cholecystokinin octapeptide;
D O I
10.1016/S0167-0115(02)00157-X
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Secretion of the gut hormone glucagon-like peptide-1 (GLP-1) is stimulated by meal ingestion. The response is rapid, suggesting a stimulatory pathway elicited from the upper gastrointestinal area. In pigs, we have been unable to demonstrate a neural stimulatory pathway, but GLP-1 secretion is regulated by local somatostatin secretion. In search for an endocrine pathway, we studied the effect of a range of concentrations of cholecystokinin octapeptide (26-33) (CCK 8), gastric inhibitory peptide 1-42 (GIP), secretin, motilin, calcitonin gene-related peptide (CGRP), and the modified amino acid, 5-hydroxytryptamine (serotonin, 5-HT) on GLP-1 and somatostatin release from isolated perfused segments of porcine ileum. GLP-1 secretion was stimulated by 1 nM CCK 8 and 10 nM GIP, but suppressed by 1 nM motilin and 1 muM 5-HT. Secretin and CGRP had no effect. Somatostatin secretion was stimulated by CCK 8 at I and 10 nM, by GIP at I and 10 nM and by 10 nM CGRP. Secretin, 5-HT and motilin had no effect on somatostatin secretion. We conclude that CCK 8 and GIP 1-42 stimulated GLP-1 secretion, but only in concentrations greatly exceeding normal postprandial concentrations. Thus, we find it unlikely that endocrine agents from the duodenum regulate GLP-1 secretion in pigs. (C) 2002 Elsevier Science B.V All rights reserved.
引用
收藏
页码:39 / 45
页数:7
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