Ultrasensitive, Multiplexed Detection of Cancer Biomarkers Directly in Serum by Using a Quantum Dot-Based Microfluidic Protein Chip

被引：227

作者：

Hu, Mei ^{[2
]}

Yan, Juan ^{[1
]}

He, Yao ^{[1
,4
]}

Lu, Haoting ^{[2
]}

Weng, Lixing ^{[3
]}

Song, Shiping ^{[1
]}

Fan, Chunhai ^{[1
]}

Wang, Lianhui ^{[2
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China

[2] Fudan Univ, Adv Mat Lab, Shanghai 200433, Peoples R China

[3] Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China

[4] Suzhou Univ, Funct Nano & Soft Mat Lab, Jiangsu 215123, Peoples R China

来源：

ACS NANO | 2010年 / 4卷 / 01期

基金：

中国国家自然科学基金;

关键词：

quantum dots; microfluidic network; protein chip; cancer biomarkers detection; multiplex target detection; CDTE NANOCRYSTALS; TUMOR VASCULATURE; DEVICE; PHASE;

D O I：

10.1021/nn901404h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sensitive and selective detection for cancer biomarkers are critical in cancer clinical-diagnostics. Here we developed a microfluidic protein chip for an ultrasensitive and multiplexed assay of cancer biomarkers. Aqueous-phase-synthesized CdTe/CdS quantum dots (aqQDs) were employed as fluorescent signal amplifiers to improve the detection sensitivity. Secondary antibodies (goat anti-mouse IgG) were conjugated to luminescent CdTe/CdS QDs to realize a versatile fluorescent probe that could be used for multiplexed detection in both sandwich and reverse phase immunoassays. We found that our microfluidic protein chip not only possessed ultrahigh femtomolar sensitivity for cancer biomarkers, but was selective enough to be directly used in serum. This protein chip thus combines the high-throughput capabilities of a microfluidic network with the high sensitivity and multicolor imaging ability offered by highly fluorescent QDs, which can become a promising diagnostic tool In clinical applications.

引用

页码：488 / 494

页数：7

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