Identification and characterization of novel cytochrome P450 genes from the white-rot basidiomycete, Coriolus versicolor

被引:22
作者
Ichinose, H [1 ]
Wariishi, H [1 ]
Tanaka, H [1 ]
机构
[1] Kyushu Univ, Fac Agr, Higashi Ku, Fukuoka 8128581, Japan
基金
日本学术振兴会;
关键词
D O I
10.1007/s00253-001-0868-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Using a reverse-transcription polymerase chain reaction (RT-PCR) technique, cytochrome 1,3450 genes were cloned from the lignin-degrading basidiomycete, Coriolus versicolor. One possible P450 gene was identified, which consisted of 1,672 nucleotides and a poly(A) tail and encoded a deduced protein containing 449 amino acids. The deduced amino acid sequence revealed the presence of the P450 heme-binding motif, strongly suggesting that this protein belongs to the P450 superfamily, then designated CYP512A1. The deduced protein showed sequential similarity to other known P450s from several micro-organisms, such as Aspergillus terreus, Gibberella fujikuroi, and Neurospora crassa, with 30-35% identity. Since the identity of the amino acid sequence was less than 40% with any other P450s, this protein was suggested to be the first member of a new family of cytochrome P450. In addition, a differential display RT-PCR analysis showed the expression of the other P450 genes, which were up-regulated by the addition of dibenzothiophene and 4-methyldibenzothiophene-5-oxide. Using the 5' rapid amplification of cDNA ends method, a 520-nucleotide sequence, including the P450 motif-coding region, was determined for one clone. The deduced protein showed high similarity to CYP512A1 but less than 40% identity with P450s from other organisms. A chemical stress-responsive expression of P450 is suggested for the first time in basidiomycetes.
引用
收藏
页码:97 / 105
页数:9
相关论文
共 33 条
[1]  
Akileswaran L, 1999, APPL ENVIRON MICROB, V65, P415
[2]   Characterization of the gene encoding manganese peroxidase isozyme 3 from Phanerochaete chrysosporium [J].
Alic, M ;
Akileswaran, L ;
Gold, MH .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1997, 1338 (01) :1-7
[3]  
[Anonymous], 1981, LIGNIN BIODEGRADATIO
[4]   Initial oxidation products in the metabolism of pyrene, anthracene, fluorene, and dibenzothiophene by the white rot fungus Pleurotus ostreatus [J].
Bezalel, L ;
Hadar, Y ;
Fu, PP ;
Freeman, JP ;
Cerniglia, CE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (07) :2554-2559
[5]  
BLACK SD, 1987, ADV ENZYMOL RAMB, V60, P35
[6]  
Eriksson K.E., 1990, MICROBIAL ENZYMATIC, P225
[7]  
FROHMAN MA, 1993, METHOD ENZYMOL, V218, P340
[8]   CHARACTERIZATION OF A GENE ENCODING A MANGANESE PEROXIDASE FROM PHANEROCHAETE-CHRYSOSPORIUM [J].
GODFREY, BJ ;
MAYFIELD, MB ;
BROWN, JA ;
GOLD, MH .
GENE, 1990, 93 (01) :119-124
[9]  
GOLD MH, 1989, ACS SYM SER, V389, P127
[10]   MOLECULAR-BIOLOGY OF THE LIGNIN-DEGRADING BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM [J].
GOLD, MH ;
ALIC, M .
MICROBIOLOGICAL REVIEWS, 1993, 57 (03) :605-622