A GABAA receptor of defined subunit composition and positioning:: Concatenation of five subunits

被引：78

作者：

Baur, R ^{[1
]}

Minier, F ^{[1
]}

Sigel, E ^{[1
]}

机构：

[1] Univ Bern, Dept Pharmacol, CH-3010 Bern, Switzerland

来源：

FEBS LETTERS | 2006年 / 580卷 / 06期

关键词：

GABA; GABA(A) receptor; benzodiazepines;

D O I：

10.1016/j.febslet.2006.02.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that the five subunits of a gamma-aminobutyric acid type A receptor (GABA(A) receptor) can be concatenated to yield a functional receptor. This concatenated receptor alpha(1)-beta(2)-alpha(1)-gamma(2)-beta(2) has the advantage of a known subunit arrangement. Most of its functional properties are not significantly different from a receptor formed by individual subunits. Extent of expression amounted to about 40% of that of non-concatenated receptors in Xenopus oocytes, after injection of oocytes with comparable amounts of cRNA coding for concatenated and non-concatenated receptors. The ability to express receptors consisting of five subunits enables detailed studies of GABAA receptor subtype selective compounds. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1616 / 1620

页数：5

共 23 条

[1] STOICHIOMETRY OF A RECOMBINANT GABA(A) RECEPTOR DEDUCED FROM MUTATION-INDUCED RECTIFICATION [J].