Retrospective detection of exposure to organophosphorus anti-cholinesterases: Mass spectrometric analysis of phosphylated human butyrylcholinesterase

被引：187

作者：

Fidder, A ^{[1
]}

Hulst, AG ^{[1
]}

Noort, D ^{[1
]}

de Ruiter, R ^{[1
]}

van der Schans, MJ ^{[1
]}

Benschop, HP ^{[1
]}

Langenberg, JP ^{[1
]}

机构：

[1] TNO, Prins Maurits Lab, Div Chem & Biol Protect, NL-2280 AA Rijswijk, Netherlands

来源：

CHEMICAL RESEARCH IN TOXICOLOGY | 2002年 / 15卷 / 04期

关键词：

D O I：

10.1021/tx0101806

中图分类号：

R914 [药物化学];

学科分类号：

100701 [药物化学];

摘要：

In this paper a novel and general procedure is presented for detection of organophosphate-inhibited human butyrylcholinesterase (HuBuChE), which is based on electrospray tandem mass spectrometric analysis of phosphylated nonapeptides obtained after pepsin digestion of the enzyme. The utility of this method is exemplified by the positive analysis of serum samples from Japanese victims of the terrorist attack with sarin in the Tokyo subway in 1995.

引用

页码：582 / 590

页数：9

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