In situ sequencing for RNA analysis in preserved tissue and cells

被引：633

作者：

Ke, Rongqin ^{[1
,2
]}

Mignardi, Marco ^{[1
,2
]}

Pacureanu, Alexandra ^{[3
]}

Svedlund, Jessica ^{[1
]}

Botling, Johan ^{[2
]}

Wahlby, Carolina ^{[3
,4
]}

Nilsson, Mats ^{[1
,2
]}

机构：

[1] Stockholm Univ, Sci Life Lab, Dept Biochem & Biophys, S-10691 Stockholm, Sweden

[2] Uppsala Univ, Dept Immunol Genet & Pathol, Rudbeck Lab, Uppsala, Sweden

[3] Uppsala Univ, Ctr Image Anal, Sci Life Lab, Uppsala, Sweden

[4] Broad Inst Harvard & Massachusetts Inst Technol, Imaging Platform, Cambridge, MA USA

来源：

NATURE METHODS | 2013年 / 10卷 / 09期

基金：

瑞典研究理事会;

关键词：

SINGLE-CELL; PADLOCK PROBES; EVOLUTION; GENOME; TUMOR; SEQ;

D O I：

10.1038/nmeth.2563

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. We have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. We demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.

引用

页码：857 / +

页数：6

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