Development of an immunomagnetic capture-reverse transcriptase-PCR assay for three pineapple ampeloviruses

被引：11

作者：

Gambley, C. F. ^{[1
,2
]}

Geering, A. D. W. ^{[1
]}

Thomas, J. E. ^{[1
]}

机构：

[1] Dept Primary Ind & Fisheries, Indooroopilly, Qld 4068, Australia

[2] Univ Queensland, Sch Integrat Biol, St Lucia, Qld 4072, Australia

来源：

JOURNAL OF VIROLOGICAL METHODS | 2009年 / 155卷 / 02期

关键词：

Semi-automation; Mealybug wilt disease; Pineapple mealybug wilt-associated virus; RT-PCR; HEPATITIS-A VIRUS; POLYMERASE-CHAIN-REACTION; HYBRIDIZATION RT-PCR; MEALYBUG WILT; GENETIC DIVERSITY; IMMUNOCAPTURE; AUSTRALIA; DISEASE; SAMPLES; BANANA;

D O I：

10.1016/j.jviromet.2008.10.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A semi-automated, immunomagneticcapture-reverse transcription PCR(IMC-RT-PCR) assay for the detection of three pineapple-infecting ampeloviruses, Pineapple mealybug wilt-associated virus-1, -2 and -3, is described. The assay was equivalent in sensitivity but more rapid than conventional immunocapture RT-PCR. The assay can be used either as a one- or two-step RT-PCR and allows detection of the viruses separately or together in a triplex assay from fresh, frozen or freeze-dried pineapple leaf tissue. This IMC-RT-PCR assay could be used for high throughput screening of pineapple planting propagules and could easily be modified for the detection of other RNA viruses in a range of plant species, provided suitable antibodies are available. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.

引用

页码：187 / 192

页数：6

共 25 条

[1] MEALYBUG WILT OF PINEAPPLE - A REAPPRAISAL [J].

CARTER, W .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1963, 105 (13) :741-&

[2] Detection of enteroviruses, hepatitis A virus and rotaviruses in sewage by means of an immunomagnetic capture reverse transcription-PCR assay [J].

Casas, N ;

Suñén, E .

MICROBIOLOGICAL RESEARCH, 2002, 157 (03) :169-175

[3] Detection of Ralstonia solanacearum in soil and weeds from commercial tomato fields using immunocapture and the polymerase chain reaction [J].

Dittapongpitch, V ;

Surat, S .

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT, 2003, 151 (04) :239-246

[4] Efficient cloning of cDNA from grapevine leafroll-associated virus 4 and demonstration of probe specificity by the viral antibody [J].

Fazeli, CF ;

Habili, N ;

Rezaian, MA .

JOURNAL OF VIROLOGICAL METHODS, 1998, 70 (02) :201-211

[5] Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR [J].

Fu, Z ;

Rogelj, S ;

Kieft, TL .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2005, 99 (01) :47-57

[6] Identification of viral and non-viral reverse transcribing elements in pineapple (Ananas comosus), including members of two new badnavirus species [J].

Gambley, C. F. ;

Geering, A. D. W. ;

Steele, V. ;

Thomas, J. E. .

ARCHIVES OF VIROLOGY, 2008, 153 (08) :1599-1604

[7] The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease [J].

Gambley, C. F. ;

Steele, V. ;

Geering, A. D. W. ;

Thomas, J. E. .

AUSTRALASIAN PLANT PATHOLOGY, 2008, 37 (02) :95-105

[8] Genetic diversity among Banana streak virus isolates from Australia [J].

Geering, ADW ;

McMichael, LA ;

Dietzgen, RG ;

Thomas, JE .

PHYTOPATHOLOGY, 2000, 90 (08) :921-927

[9]

ITO K, 1959, TERMINAL MOTTLE SYMP, P1

[10]

Jothikumar N, 1998, APPL ENVIRON MICROB, V64, P504

← 1 2 3 →