Identification of PTEN-related sequences in glioma cells and in non-neoplastic cell lines

被引：4

作者：

Chiariello, E

Roz, L

Albarosa, R

Magnani, I

Finocchiaro, G

机构：

[1] Ist Nazl Neurol Carlo Besta, Lab NeuroOncol & Gene Therapy, I-20133 Milan, Italy

[2] Univ Milan, Dept Biol & Genet Med Sci, I-20122 Milan, Italy

来源：

CANCER LETTERS | 1999年 / 138卷 / 1-2期

关键词：

glioma; tumor suppressor gene; pseudogene;

D O I：

10.1016/S0304-3835(98)00357-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The PTEN gene, which encodes a tumor suppressor with phosphatase activity, is located on chromosome 10q23 and is mutated in different tumors, including glioblastomas (GBM). We found evidence for a PTEN-related sequence (PTEN-rs) on genomic DNA of GEM and non-neoplastic cells. PTEN-rs does not contain introns and presents several conserved missense mutations, including a T to G transversion at the initiation codon. Rsa I digestion may help to identify this putative PTEN pseudogene which, according to RT-PCR analysis on glioma, fibroblast, brain and lung cells, does not appear to be transcribed. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

收藏

页码：1 / 4

页数：4

相关论文

共 15 条

[1]

Albarosa R, 1996, AM J HUM GENET, V58, P1260

[2] PTEN/MMAC1 mutations in primary glioblastomas and short-term cultures of malignant gliomas [J].

Chiariello, E ;

Roz, L ;

Albarosa, R ;

Magnani, I ;

Finocchiaro, G .

ONCOGENE, 1998, 16 (04) :541-545

[3] Growth suppression of glioma cells by PTEN requires a functional phosphatase catalytic domain [J].

Furnari, FB ;

Lin, H ;

Huang, HJS ;

Cavenee, WK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (23) :12479-12484

[4] Alterations of PTEN/MMAC1, a candidate tumor suppressor gene, and its homologue, PTH2, in small cell lung cancer cell lines [J].

Kim, SK ;

Su, LK ;

Oh, Y ;

Kemp, BL ;

Hong, WK ;

Mao, L .

ONCOGENE, 1998, 16 (01) :89-93

[5]

Li DM, 1997, CANCER RES, V57, P2124

[6] PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer [J].

Li, J ;

Yen, C ;

Liaw, D ;

Podsypanina, K ;

Bose, S ;

Wang, SI ;

Puc, J ;

Miliaresis, C ;

Rodgers, L ;

McCombie, R ;

Bigner, SH ;

Giovanella, BC ;

Ittmann, M ;

Tycko, B ;

Hibshoosh, H ;

Wigler, MH ;

Parsons, R .

SCIENCE, 1997, 275 (5308) :1943-1947

[7] TENSIN - A POTENTIAL LINK BETWEEN THE CYTOSKELETON AND SIGNAL-TRANSDUCTION [J].

LO, SH ;

WEISBERG, E ;

CHEN, LB .

BIOESSAYS, 1994, 16 (11) :817-823

[8] INCREASING COMPLEXITY OF THE KARYOTYPE IN 50 HUMAN GLIOMAS - PROGRESSIVE EVOLUTION AND DE-NOVO OCCURRENCE OF CYTOGENETIC ALTERATIONS [J].

MAGNANI, I ;

GUERNERI, S ;

POLLO, B ;

CIRENEI, N ;

COLOMBO, BM ;

BROGGI, G ;

GALLI, C ;

BUGIANI, O ;

DIDONATO, S ;

FINOCCHIARO, G ;

CONTI, AMF .

CANCER GENETICS AND CYTOGENETICS, 1994, 75 (02) :77-89

[9] P-TEN, the tumor suppressor from human chromosome 10q23, is a dual-specificity phosphatase [J].

Myers, MP ;

Stolarov, JP ;

Eng, C ;

Li, J ;

Wang, SI ;

Wigler, MH ;

Parsons, R ;

Tonks, NK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (17) :9052-9057

[10]

Rasheed BKA, 1997, CANCER RES, V57, P4187