Preparative electrophoresis: a general method for the purification of polyclonal antibodies

被引：28

作者：

Thomas, TM ^{[1
]}

Shave, EE ^{[1
]}

Bate, IM ^{[1
]}

Gee, SC ^{[1
]}

Franklin, S ^{[1
]}

Rylatt, DB ^{[1
]}

机构：

[1] Gradipore Ltd, Frenchs Forest, NSW 2086, Australia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 944卷 / 1-2期

关键词：

preparative electrophoresis; instrumentation; Gradiflow; antibodies; immunoglobulins;

D O I：

10.1016/S0021-9673(01)01283-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibodies were purified from normal rabbit, sheep, goat, rat, human and bovine serum using preparative electrophoresis on a Gradiflow in a single-step process using an asymmetrical cartridge with three different pore size polyacrylamide membranes. Recoveries in each case were over 80% and were higher than those obtained using affinity chromatography on protein A, protein G or protein L. Degree of purity was at least comparable with these methods. These results suggest that preparative electrophoresis can be considered a general method for the purification of research quantities of antibodies from multiple serum sources and may be particularly useful where the reactivity with protein A, G or L is unknown. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：161 / 168

页数：8

共 42 条

[1] ON THE INTERACTION BETWEEN SINGLE-CHAIN FV ANTIBODIES AND BACTERIAL IMMUNOGLOBULIN-BINDING PROTEINS [J].