The structure of intramolecular triplex DNA: Atomic force microscopy study

被引：54

作者：

Tiner, WJ

Potaman, VN

Sinden, RR

Lyubchenko, YL ^{[1
]}

机构：

[1] Arizona State Univ, Dept Microbiol, Tempe, AZ 85281 USA

[2] Texas A&M Univ Syst, Inst Biosci & Technol, Hlth Sci Ctr, Houston, TX 77030 USA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2001年 / 314卷 / 03期

关键词：

atomic force microscopy; intramolecular triplexes; alternative DNA structures; H-DNA; DNA supercoiling;

D O I：

10.1006/jmbi.2001.5174

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We applied atomic force microscopy (ATM) for direct imaging of intramolecular triplexes (H-DNA) formed by mirror-repeated purine-pyrimidine repeats and stabilized by negative DNA supercoiling. H-DNA appears in atomic force microscopy images as a clear protrusion with a different thickness than DNA duplex. Consistent with the existing models, H-DNA formation results in a kink in the double helix path. The kink forms an acute angle so that the flanking DNA regions are brought in close proximity. The mobility of flanking DNA arms is limited compared with that for cruciforms and three-way junctions. Structural properties of H-DNA may be important for promoter-enhancer interactions and other DNA transactions. (C) 2001 Academic Press.

引用

页码：353 / 357

页数：5

共 25 条

[1] AN OVERABUNDANCE OF LONG OLIGOPURINE TRACTS OCCURS IN THE GENOME OF SIMPLE AND COMPLEX EUKARYOTES [J].