Optimisation of aqueous two-phase extraction of anthocyanins from purple sweet potatoes by response surface methodology

被引：140

作者：

Liu, Xingli ^{[1
]}

Mu, Taihua ^{[1
]}

Sun, Hongnan ^{[1
]}

Zhang, Miao ^{[1
]}

Chen, Jingwang ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Key Lab Agroprod Proc, Minist Agr, Beijing 100193, Peoples R China

来源：

FOOD CHEMISTRY | 2013年 / 141卷 / 03期

关键词：

Anthocyanins; Purple sweet potato; Aqueous two-phase; Response surface methodology; ASSISTED EXTRACTION; ANTIOXIDANT ACTIVITY; PHENOLIC-COMPOUNDS; MASS-SPECTROMETRY; NATURAL COLORANTS; SYSTEMS; CHROMATOGRAPHY; PARTITION; RECOVERY; SEEDS;

D O I：

10.1016/j.foodchem.2013.05.119

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Aqueous two-phase extraction (ATPE) method was investigated for extraction of anthocyanins from purple sweet potatoes using response surface methodology (RSM). Results showed that the optimal conditions for anthocyanin extraction were that, 45:1 (mL/g) liquid-solid ratio, 25% (W/W) ethanol, 22% (W/W) concentration of ammonium sulphate and pH3.3; the anthocyanin yield and partition coefficient under the optimal conditions were 90.02% and 19.62, respectively. The result of HPLC-ESI-MS analysis revealed eight kinds of compounds, and the major anthocyanins as cyanidi-caffeoy-fumaroy-sophoroside-3-O-glucoside, peonidin-caffeoyl-hydroxybenzoyl-3-O-glucoside, peonidin-caffeoyl-sophoroside-3-O-glucoside, and peonidin-caffeoyl-fumaroyl-sophorosid-3-O-glucoside. Meanwhile, we found a compound as a dimer of galloyl procyanin. These results suggest that ATPE is efficient in extracting anthocyanins and has the potential to be used in natural anthocyanin extraction industry. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：3034 / 3041

页数：8

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