Crystallization and preliminary X-ray analysis of bacteriophage lambda lysozyme in which all tryptophans have been replaced by aza-tryptophans

After many unsuccessful attempts to crystallize the bacteriophage lambda lysozyme, a mutant where all the tryptophan residues have been replaced by aza-tryptophans has been crystallized by the vapor-diffusion method. The crystals are orthorhombic and belong to space group P2(1)2(1)2(1) With cell dimensions a = 73.01, b = 78.80, c = 82.31 Angstrom. Diffraction data were collected using synchrotron radiation sources. Crystals diffract to a resolution of 2.3 Angstrom. Data from two different platinum derivatives were also recorded to 2.8 and 2.5 Angstrom, respectively.