Combined method for the determination of γ-aminobutyric and β-alanine in cerebrospinal fluid by stable isotope dilution mass spectrometry

被引：11

作者：

Struys, EA ^{[1
]}

Guérand, WS ^{[1
]}

ten Brink, HJ ^{[1
]}

Jakobs, C ^{[1
]}

机构：

[1] Free Univ Amsterdam Hosp, Dept Clin Chem & Pediat, NL-1081 HV Amsterdam, Netherlands

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 732卷 / 01期

关键词：

stable isotope dilution; gamma-aminobutyric acid; beta-alanine;

D O I：

10.1016/S0378-4347(99)00283-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A previously described method for the determination of GABA in CSF has been expanded to include both GABA and beta-ALA, using a single GC-MS analysis. A stable isotope labelled internal standard for beta-ALA was synthesised to achieve accurate quantification. This new combined method expands the diagnostic power compared to an isolated GABA measurement. Control values for free and total GABA and free and total beta-ALA are described. Age <2 years: free GABA 0.017-0.067 mu M. total GABA 4.2-13.4 mu M; free beta-ALA 0.049-0.11 mu M. total beta-ALA 2.1-4.6 mu M Age >2 years: free GABA 0.032-0.17 mu M, total GABA 3.3-12.2 mu M; free beta-ALA 0.021-0.058 mu M. total beta-ALA 0.91-3.5 mu M (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：245 / 249

页数：5

共 9 条

[1]

DA M, 1999, J CHROMATOGR B, V726, P285

[2]

GIBSON KM, IN PRESS J INHER MET

[3] PYRIDOXINE-RESPONSIVE HYPER-BETA-ALANINEMIA ASSOCIATED WITH COHENS SYNDROME [J].

HIGGINS, JJ ;

KANESKI, CR ;

BERNARDINI, I ;

BRADY, RO ;

BARTON, NW .

NEUROLOGY, 1994, 44 (09) :1728-1732

[4] NORMAL AND ABNORMAL CENTRAL-NERVOUS-SYSTEM GABA-METABOLISM IN CHILDHOOD [J].

JAEKEN, J ;

CASAER, P ;

HAEGELE, KD ;

SCHECHTER, PJ .

JOURNAL OF INHERITED METABOLIC DISEASE, 1990, 13 (06) :793-801

[5] STABLE-ISOTOPE DILUTION ANALYSIS OF GABA IN CSF USING SIMPLE SOLVENT-EXTRACTION AND ELECTRON-CAPTURE NEGATIVE-ION MASS FRAGMENTOGRAPHY [J].

KOK, RM ;

HOWELLS, DW ;

VANDENHEUVEL, CCM ;

GUERAND, WS ;

THOMPSON, GN ;

JAKOBS, C .

JOURNAL OF INHERITED METABOLIC DISEASE, 1993, 16 (03) :508-512

[6] RAPID ASSAY FOR GAMMA-AMINOBUTYRIC-ACID IN MOUSE-BRAIN SYNAPTOSOMES USING GAS-CHROMATOGRAPHY MASS-SPECTROMETRY [J].

PALATY, J ;

BURTON, R ;

ABBOTT, FS .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 662 (01) :85-90

[7] Inherited disorders of GABA metabolism [J].

Pearl, Phillip L. ;

Hartka, Thomas R. ;

Cabalza, Jessica L. ;

Taylor, Jacob ;

Gibson, Michael .

FUTURE NEUROLOGY, 2006, 1 (05) :631-636

[8] IMPROVED SYNTHESIS OF 5-FLUOROURACIL-6-C-14 [J].

PERRY, CW ;

BURGER, W ;

BADER, GJ ;

LIEBMAN, AA .

JOURNAL OF LABELLED COMPOUNDS, 1975, 11 (04) :583-589

[9] 2',3',5'-TRI-O-BENZOYL[4-C-13]URIDINE - EFFICIENT, REGIOSPECIFIC SYNTHESIS OF PYRIMIDINE RING [J].

ROBERTS, JL ;

POULTER, CD .

JOURNAL OF ORGANIC CHEMISTRY, 1978, 43 (08) :1547-1555

← 1 →