Complementation analysis in PtdInsP kinase-deficient yeast mutants demonstrates that Schizosaccharomyces pombe and murine Fab1p homologues are phosphatidylinositol 3-phosphate 5-kinases

Phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P-2) is widespread in eukaryotic cells. In Saccharomyces cerevisiae, PtdIns(3,5)P-2 synthesis is catalyzed by the PtdIns3P 5 kinase Fab1p, and loss of this activity results in vacuolar morphological defects, indicating that PtdIns(3,5)P-2 is essential for vacuole homeostasis, We have therefore suggested that all Fab1p homologues may be PtdIns3P Ei-kinases involved in membrane trafficking. It is unclear which phosphatidylinositol phosphate kinases (PIPkins) are responsible for PtdIns(3,5)P-2 synthesis in higher eukaryotes. To clarify how PtdIns(3,5)P-2 is synthesized in mammalian and other cells, we determined whether yeast and mammalian Fab1p homologues or mammalian Type I PIPkins (PtdIns4P B-kinases) make Ptdins(3,5)P-2 in vivo. The recently cloned murine (p235) and Schizosaccharomyces pombe FAB1 homologues both restored basal PtdIns(3,5)P-2 synthesis in Delta fab1 cells and made PtdIns(3,5)P-2 in vitro. Only p235 corrected the grow-th and vacuolar defects of fab1 S. cerevisiae, A mammalian Type I PIPkin supported no PtdIns(3,5)P-2 synthesis. Thus, FAB1 and its homologues constitute a distinct class of Type III PIPkins dedicated to PtdIns(S,5)P-2 synthesis, The differential abilities of p235 and of SpFab1p to complement the phenotypic defects of Delta fab1 cells suggests that interaction(s) with other protein factors may be important for spatial and/or temporal regulation of PtdIns(3,5)P-2 synthesis. These results also suggest that p235 may regulate a step in membrane trafficking in mammalian cells that is analogous to its function in yeast.